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An “off–on” electrochemiluminescence aptasensor for determination of lincomycin based on CdS QDs/carboxylated g-C3N4

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Abstract

A novel electrochemiluminescence (ECL) aptasensor for the determination of lincomycin (LIN) was developed based on CdS QDs/carboxylated g-C3N4 (CdS QDs/C-g-C3N4). CdS QDs/C-g-C3N4 served as the substrate of the aptasensor, and then CdS QDs/C-g-C3N4-modified electrode was incubated with aptamer DNA (Apt-DNA). When the non-specific sites of the electrode surface was blocked by 6-mercaptohexanol, the ferrocene-labeled probe (Fer-DNA) was assembled onto the electrode surface through base complementation with Apt-DNA. In the absence of LIN, the ECL signal was quenched effectively by Fer-DNA and a decreased ECL emission (off state) was acquired. On the contrary, LIN was specifically bond with Apt-DNA, and Fer-DNA was detached from the aptasensor surface because of the deformation of Apt-DNA, resulting in an effectively enhanced ECL signal (on state). The constructed ECL aptasensor exhibited a wide detection range for LIN determination (0.05 ng mL−1–100 μg mL−1) with a low detection limit (0.02 ng mL−1). Importantly, the proposed ECL aptasensor showed outstanding accuracy and specificity for LIN determination, and also provided a potential strategy for other antibiotic determinations.

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All data generated or analysed during this study are included in this published article (and its supplementary information files).

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Funding

This work was financially supported by the National Natural Science Foundation of China (No. 22104030), Natural Science Foundation of Henan (No 222300420426) and the Innovative Funds Plan of Henan University of Technology (No. 2020ZKCJ03).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, People’s Republic of China

    Yunfeng Fan, Zhimin Liu, Jie Wang, Chen Cui & Leqian Hu

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  1. Yunfeng Fan
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  2. Zhimin Liu
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  3. Jie Wang
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  4. Chen Cui
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  5. Leqian Hu
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Correspondence to Zhimin Liu.

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Fan, Y., Liu, Z., Wang, J. et al. An “off–on” electrochemiluminescence aptasensor for determination of lincomycin based on CdS QDs/carboxylated g-C3N4. Microchim Acta 190, 11 (2023). https://doi.org/10.1007/s00604-022-05587-w

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