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2D Co3O4 modified by IrO2 nanozyme for convenient detection of aqueous Fe2+ and intercellular H2O2

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Abstract

A portable sensor for visual monitoring of Fe2+ and H2O2, two-dimensional Co3O4 modified by nano-IrO2 (IrO2@2D Co3O4) was prepared in this work, for the first time, with the help of microwave radiation at 140 °C, which was further stabilized onto common test strips. The present IrO2@2D Co3O4 possessed superior dual-function enzyme-like activity with low toxicity and excellent biocompatibility. Especially, trace Fe2+ and H2O2 could exclusively alter their enzyme-like catalytic activity with discriminating hyperchromic or hypochromic effect, i.e., from blue to colorless or to dark blue for both IrO2@2D Co3O4 dispersion and its functionalized test strips. The linear regression equations were A652 = 0.5940 − 0.00041 cFe2+ (10−8 M, R2 = 0.9927) for Fe2+ and ∆A652 = 0.0023 cH2O2 + 0.00025 (10−7 M, R2 = 0.9982) for H2O2, respectively. When applied to visual monitoring of aqueous Fe2+ and intercellular H2O2, the recoveries were 101.2 ~ 102.5% and 95.8 ~ 103.7% with detection limits of 1.25 × 10−8 mol/L and 1.02 × 10−7 mol/L, respectively, far below the permitted values in drinking water set by the World Health Organization. The mechanisms for the enhancing enzyme-mimetic activity of IrO2@2D Co3O4 and its selective responses to Fe2+ and H2O2 were investigated in detail.

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Funding

The authors gratefully acknowledge the financial support from the National Natural Science Foundation of China (Nos.: 22276108, 51773171, 12074322, and 21806089) and Natural Science Foundation of Shandong Province (Nos.: ZR2021MB002 and ZR2018MB038).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering & Shandong Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, 273165, China

    Zhengquan Yan, Xiaoyu Zheng, Yulian Tang, Xuemei Zhou, Junkai Hao, Jing Feng & Lei Hu

  2. Fujian Provincial Key Laboratory for Soft Functional Materials Research &, School of Material Science and Engineering, Xiamen University, Xiamen, 237012, China

    Guoqing Meng & Naibo Lin

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  1. Zhengquan Yan
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  2. Xiaoyu Zheng
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Yan, Z., Zheng, X., Meng, G. et al. 2D Co3O4 modified by IrO2 nanozyme for convenient detection of aqueous Fe2+ and intercellular H2O2. Microchim Acta 190, 1 (2023). https://doi.org/10.1007/s00604-022-05582-1

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