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A sensitive multimode dot-filtration strip for the detection of Salmonella typhimurium using MoS2@Fe3O4

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Abstract

A sensitive, accurate, and rapid multimode dot-filtration immunoassay (MDFIA) was established for the detection of Salmonella typhimurium using the intrinsic color, catalytic property, and photothermal effect of magnetic molybdenum disulphide (MoS2@Fe3O4). The critical performance parameters of MDFIA were optimized in detail. The sensitivity of MDFIA can be improved by the catalytic color development and photothermal conversion of MoS2@Fe3O4 with a limit of detection (LOD) of 101 CFU·mL−1, which is an order of magnitude lower than direct visual detection (102 CFU·mL−1). Besides, the magnetic property of MoS2@Fe3O4 was used for the rapid enrichment and separation of the target allowing detection of trace concentrations of Salmonella typhimurium. The selectivity and applicability of the MDFIA were verified in spiked samples, indicating that the established assay may have bright application prospects for the detection and control of foodborne pathogens.

Graphical abstract

A multimode dot-filtration immunoassay was constructed for Salmonella typhimurium rapid detection based on the peroxidase-like activity, magnetic property, and photothermal effect of MoS2@Fe3O4.

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Abbreviations

MoS2@Fe3O4 :

Magnetic molybdenum disulphide nanoparticles

MDFIA:

Multimode dot-filtration immunoassay

ELISA:

Enzyme-linked immunosorbent assay

IFTS:

Immunofiltration test strips

POCT:

Point-of-care testing

SPR:

Surface plasmon resonance

L-Cys:

L-Cysteine

BSA:

Bovine serum albumin

NCM:

Nitrocellulose membrane

ΔT :

Temperature change value

TMB:

3,3′,5,5′-Tetramethylbenzidine

PBS:

Phosphate buffer solution

MoS2@Fe3O4-L-Cys:

MoS2@Fe3O4 modified with L-Cysteine

DLS:

Dynamic light scattering

TEM:

Transmission electron microscopy

XRD:

X-ray diffraction

NIR:

Near-infrared

K m :

Michaelis-Menten constant

LOD:

Limit of detection

CV:

Coefficient of variation

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Funding

This work was supported by the National Natural Science Foundation of China (31871874) and the Natural Science Foundation of Shandong Province (ZR2020KC031, ZR2021MC132).

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Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan, 250014, People’s Republic of China

    Luxiang Gao, Xiaoyu Xu, Wenxiu Liu, Jinjuan Xie, Hongyan Zhang & Shuyuan Du

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  1. Luxiang Gao
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  2. Xiaoyu Xu
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  4. Jinjuan Xie
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  5. Hongyan Zhang
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Contributions

Conceptualization: Shuyuan Du; data curation: Luxiang Gao; formal analysis: Luxiang Gao, Xiaoyu Xu, Wenxiu Liu, Jinjuan Xie, Shuyuan Du; funding acquisition: Hongyan Zhang; investigation: Luxiang Gao, Wenxiu Liu; methodology: Hongyan Zhang, Shuyuan Du; project administration: Hongyan Zhang; resources: Hongyan Zhang; supervision: Luxiang Gao; validation: Luxiang Gao, Xiaoyu Xu, Wenxiu Liu, Jinjuan Xie; visualization: Luxiang Gao; writing—original draft: Luxiang Gao; writing—review and editing: Shuyuan Du.

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Correspondence to Hongyan Zhang or Shuyuan Du.

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Gao, L., Xu, X., Liu, W. et al. A sensitive multimode dot-filtration strip for the detection of Salmonella typhimurium using MoS2@Fe3O4. Microchim Acta 189, 475 (2022). https://doi.org/10.1007/s00604-022-05560-7

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