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Colorimetric determination of biothiols based on peroxidase-mimicking Ag nanoparticles decorated Ti3C2 nanosheets

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Abstract 

Ag nanoparticle–decorated Ti3C2 nanosheets (AgNPs@Ti3C2 NSs) were facilely synthesized via a self-reduction approach, in which Ti3C2 NSs acted as both reductant and supporter. The AgNPs@Ti3C2 NS nanocomposite exhibited excellent peroxidase-like activity with o-phenylenediamine (OPD) and H2O2 as substrates. The catalytic behavior followed the typical Michaelis–Menten kinetics; Michaelis constant (Km) and maximum initial velocity (Vmax) for OPD were 0.263 mM and 43.2 × 10–8 M−1 s, indicating high affinity and high catalytic efficiency towards OPD. The catalytic mechanism was revealed to be an accelerated electron transfer process. Based on the inhibition effect on the peroxidase-like activity of AgNPs@Ti3C2 NSs, a simple, fast, and sensitive colorimetric method for detection of low-weight biothiols (cysteine (Cys), homocysteine (Hcy), and glutathione (GSH)) was developed by measuring the absorbance at 425 nm. The colorimetric method displayed wide linear range (50 nM to 50 μM for Cys, 10 nM to 250 μM for Hcy, 10 nM to 50 μM for GSH), low limit of detection (48.5 nM for Cys, 5.5 nM for Hcy, 7.0 nM for GSH), and good selectivity and short assay time (3 min). Moreover, the feasibility of this colorimetric sensor was demonstrated by accurately determining Cys in diluted human serum samples; good recovery (95.9–101.0%) and low relative standard deviations (2.8–4.9%) were obtained, showing great promise for point-of-care test in clinical samples.

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Funding

This work was financially supported by National Natural Science Foundation of China (Grant no. 62001335), Young and Middle-Aged Talent Program of Hubei Provincial Department of Education (Q20201508), and Scientific Research Foundation of Wuhan Institute of Technology (K201940).

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Authors and Affiliations

  1. School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan, 430205, People’s Republic of China

    Zhen Lei, Jingfang Guo & Jing Zou

  2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, People’s Republic of China

    Zhenxin Wang

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  1. Zhen Lei
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Lei, Z., Guo, J., Zou, J. et al. Colorimetric determination of biothiols based on peroxidase-mimicking Ag nanoparticles decorated Ti3C2 nanosheets. Microchim Acta 189, 369 (2022). https://doi.org/10.1007/s00604-022-05472-6

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