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Nanozyme-based cascade SPR signal amplification for immunosensing of nitrated alpha-synuclein

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Abstract

A self-assembled nanozyme of iron porphyrin mediated supramolecular modified gold nanoparticles (FpA) was fabricated to determine nitrated alpha-synuclein as the Tyr 39 residue (nT39 α-Syn) of a potential biomarker for early diagnosis of Parkinson’s disease (PD). Mechanically, localized surface plasmon resonance (LSPR) and the mass effect caused by catalytic deposition of the nanozyme contributed to a cascade signal amplification strategy. The sensor allowed a signal amplification and selective nT39 α-Syn bioanalysis with a 1.34-fold enhancement by cascade amplified SPR signal and double specific recognition. The detection limit was 1.78 ng/mL in the detection range of 7–240 ng/mL. Benefiting from the excellent immunosensor, this method can distinguish healthy people and PD patients using actual samples. Overall, this strategy provides a nanozyme-based biosensing platform for the early diagnosis of PD and can be applied to detect other protein biomarkers, such as PD-L1.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (grant no. 62005156 and 61875114).

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Author notes

  1. Xiaojun Hu and Ruhui Hu contributed equally to this work.

Authors and Affiliations

  1. School of Life Sciences, Shanghai University, Shanghai, 200444, People’s Republic of China

    Xiaojun Hu, Ruhui Hu, Han Zhu, Qiang Chen, Yongkai Lu, Jie Chen & Hongxia Chen

  2. School of Medicine, Shanghai University, Shanghai, 200444, People’s Republic of China

    Qiang Chen, Jie Chen & Yawen Liu

  3. School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, People’s Republic of China

    Yawen Liu

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  1. Xiaojun Hu
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  2. Ruhui Hu
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Contributions

Xiaojun Hu: Conceptualization, formal analysis, and writing—original draft. Ruhui Hu: Formal analysis, validation, and writing—review and editing. Han Zhu: Formal analysis, validation, and writing—original draft. Qiang Chen: Validation, writing—original draft. Yongkai Lu: Formal analysis, writing—review and editing. Jie Chen: Data curation and writing–review and editing. Yawen Liu: Data curation and writing—review and editing. Hongxia Chen: Conceptualization, project administration, and funding acquisition.

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Correspondence to Hongxia Chen.

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Hu, X., Hu, R., Zhu, H. et al. Nanozyme-based cascade SPR signal amplification for immunosensing of nitrated alpha-synuclein. Microchim Acta 189, 367 (2022). https://doi.org/10.1007/s00604-022-05465-5

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