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Highly stable acetylcholinesterase electrochemical biosensor based on polymerized ionic liquids microgel for pesticides detection

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Abstract

A highly stable electrochemical biosensor for pesticide detection was developed. For the first time polymeric ionic liquids (PILs) were introduced to construct an acetylcholinesterase (AChE) biosensor . AChE was entrapped in PILs microspheres through an emulsion polymerization reaction, where negatively charged Au nanoparticles (Au NPs) can be immobilized by the positively charged PILs, leading to improved catalytic performance. The results suggest that the positively charged PILs not only provide a biocompatible microenvironment around the enzyme molecule, stabilizing its biological activity and preventing its leakage, but also act as a modifiable interface allowing other components with electron transport properties to be loaded onto the polymer substrate, thus providing an efficient electron transport channel for the entrapped enzyme. More notably, when AChE was immobilized in a positively charged environment, the active site is closer to the electrode, promoting faster electron transfer. The detection limits of the constructed electrochemical biosensor AChE@PILs@Au NPs/GCE toward carbaryl and dichlorvos (DDVP) were 5.0 × 10−2 ng ml−1 and 3.9 × 10−2 ng ml−1, in a wide linear range of 6.3 × 10−2–8.8 × 102 ng ml−1 and 1.3 × 10−1–1.4 × 103 ng ml−1, respectively. More importantly, the biosensor has high thermal and storage stability, which facilitates rapid field analysis of fruits and vegetables in a variety of climates. In addition, the biosensor reported has good repeatability and selectivity and has high accuracy in the analysis of peaches, tap water, and other types of samples.

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Acknowledgements

Authors Yu Wan, Huiting Wang Yaxian Chen, Shun Li, Jie Zhou, and Lixin Xia* received funding from the National Natural Science Foundation of China (21671089), the Liaoning Revitalization Talents Program (XLYC2002097), the Scientific Research Fund of Liaoning Provincial Education Department (L2020002), and the Liaoning Provincial Natural Science Foundation (2020-YKLH-22). Authors Yu Wan, Huiting Wang, Ling Zhang, Yaxian Chen, Shun Li, Jie Zhou, and Qian Zhang* received funding from the Scientific Research Fund of Liaoning Provincial Education Department (LR2019029).

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Authors and Affiliations

  1. College of Chemistry, Liaoning University, Shenyang, 110036, China

    Yu Wan, Huiting Wang, Yaxian Chen, Shun Li, Jie Zhou & Qian Zhang

  2. College of Chemistry and Chemical Engineering, Shenyang Normal University, Shenyang, 110034, China

    Ling Zhang

  3. Yingkou Institute of Technology, Yingkou, 115014, China

    Lixin Xia

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  1. Yu Wan
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Wan, Y., Wang, H., Zhang, L. et al. Highly stable acetylcholinesterase electrochemical biosensor based on polymerized ionic liquids microgel for pesticides detection. Microchim Acta 189, 300 (2022). https://doi.org/10.1007/s00604-022-05383-6

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