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CuCo2S4 nanozyme-based stimulus-responsive hydrogel kit for rapid point-of-care testing of uric acid

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Abstract

An efficient analysis platform composed of nanozyme-based hydrogel kit and smartphone was constructed for on-site detection of uric acid (UA) in a rapid and realiable manner. CuCo2S4 nanoparticles (CuCo2S4 NPs) as a peroxidase mimic were successfully prepared and the peroxidase-like activity and catalytic mechanism were studied in detail. The hydrogen peroxide (H2O2) stimulus-responsive nanozyme-based hydrogel kit was manufactured by integrating agarose, CuCo2S4 NPs, and 3,3′,5,5′-tetramethylbenzidine (TMB) into the cap of centrifuge tube. H2O2 generated via UA oxidation acts as stimulus signal, which triggers the oxidation of TMB to form blue product (oxTMB) under the catalysis of CuCo2S4 NPs, resulting in the color response of the constructed kit. The color image of the kit was captured by a smartphone built-in camera and converted into color intensity using ImageJ software, thus achieving the quantitative determination of UA. The portable kit possesses high selectivity and was used to monitor UA in human serum with satisfactory results (recovery was in the range 95.8–107.3% and RSD was not greater than 4.6%). The established sensing platform is convenient and reliable, which provides a new strategy for point-of-care testing of UA and has a broad prospect in the fields of chemical sensing and biomedical.

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Funding

This work was supported by the Middle-aged Backbone Personnel Training Program of Shenyang Pharmaceutical University (ZQN2016011), Scientific Research Fund of Liaoning Provincial Education Department (2020LZD02), Inter-school Cooperation Project of General Undergraduate Universities in Liaoning Province (2020–181), Project of Shenyang Key Laboratory of Functional Drug Carrier Materials (grant no. 19–110-4–08).

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Authors and Affiliations

  1. School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe District, Shenyang, Liaoning Province, 110016, People’s Republic of China

    Xucan Yuan, Hanqing Zhao, Yue Yuan, Mengying Chen, Longshan Zhao & Zhili Xiong

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  1. Xucan Yuan
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Correspondence to Longshan Zhao or Zhili Xiong.

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Yuan, X., Zhao, H., Yuan, Y. et al. CuCo2S4 nanozyme-based stimulus-responsive hydrogel kit for rapid point-of-care testing of uric acid. Microchim Acta 189, 283 (2022). https://doi.org/10.1007/s00604-022-05381-8

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