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Boron carbon oxynitride quantum dots-based ratio fluorescent nanoprobe assisted with smartphone for visualization detection of phosphate

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Abstract

A ratio fluorescence nanoprobe was constructed by simple mixing BCNO QDs with 8-hydroxyquinoline-5-sulfonic acid (HQSA), which had an obvious fluorescence peak at 420 nm and a weak fluorescence peak at 500 nm, corresponding to the BCNO QDs and HQSA, respectively. This fluorescence probe takes stable fluorescence of BCNO QDs as an internal standard, based on HQSA chelating enhanced fluorescence and specificity of phosphate in the presence of Mg2+, which realizes a rapid and sensitive detection of phosphate with good linearity in the range 0.3–50 μM and 50–100 μM and a detection limit of 0.073 μM. The recovery is between 94.1 and 111% and the relative standard deviations (RSDs) below 10%. At the same time, we took color photos of the reaction solution under 310-nm UV lamp with smartphones for visual detection through RGB data image analysis, which make the detection easier and faster. The proposed method provides a new strategy for the intelligent online detection of other targets in complex environment samples.

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Funding

This work was financially supported by National Natural Science Foundation of China (21707030 and 82073608).

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Authors and Affiliations

  1. Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China

    Ying Qin, Yu He & Gongwu Song

  2. School of Public Health, North China University of Science and Technology, No.21 Bohai Road, Caofeidian, Tangshan, 063210, Hebei, China

    Ziling Li & Manman Wang

Authors
  1. Ying Qin
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  2. Ziling Li
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  3. Yu He
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Correspondence to Yu He or Manman Wang.

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Qin, Y., Li, Z., He, Y. et al. Boron carbon oxynitride quantum dots-based ratio fluorescent nanoprobe assisted with smartphone for visualization detection of phosphate. Microchim Acta 189, 238 (2022). https://doi.org/10.1007/s00604-022-05331-4

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