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Fluorescence determination of glyphosate based on a DNA-templated copper nanoparticle biosensor

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Abstract

A rapid and convenient fluorescence glyphosate (GLYP) biosensor was developed based on DNA-templated copper nanoparticles (DNA-CuNPs). In the absence of GLYP, the DNA-CuNPs were formed through the reduction of Cu2+ by vitamin C (Vc). The DNA-CuNPs emitted intense fluorescence at 615 nm when being excited at 340 nm. In the presence of GLYP, GLYP can strongly chelate with Cu2+ by the phosphate and carboxyl groups to decrease the amount of free Cu2+. Due to the lack of free Cu2+, DNA-CuNPs cannot be formed, which caused the fluorescence to decrease. The whole detection process of this proposed GLYP biosensor can be completed within 14 min. Titration experiments showed that this biosensor had a linear relationship for GLYP in the range 1 to 18 µM with a limit of detection (LOD) of 0.47 µM. This biosensor showed obvious selectivity among other pesticides, even between GLYP and organophosphorus pesticides. This biosensor performed well for GLYP detection in real samples with recoveries of 88.0–104.0%.

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Funding

The work is supported by the National Natural Science Foundation of China (31601536, 31871888), and State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil & Water Pollution (GHBK-2020–001).

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Author notes

  1. Huajuan Fang and Xingping Zhang contributed equally to this work.

Authors and Affiliations

  1. College of Life Science, Yangtze University, 266 Jingmi Road, Jingzhou, 434025, Hubei, China

    Huajuan Fang, Xingping Zhang, Dongxu Gao, Yao Xiao, Liyuan Ma, Hualin Yang & Yu Zhou

  2. State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China

    Xingping Zhang

  3. State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil &Water Pollution, Chengdu University of Technology, Chengdu, 610059, Sichuan, China

    Hualin Yang

  4. College of Animal Science, Yangtze University, 266 Jingmi Road, Jingzhou, 434025, Hubei, China

    Yu Zhou

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  1. Huajuan Fang
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Correspondence to Hualin Yang or Yu Zhou.

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Fang, H., Zhang, X., Gao, D. et al. Fluorescence determination of glyphosate based on a DNA-templated copper nanoparticle biosensor. Microchim Acta 189, 158 (2022). https://doi.org/10.1007/s00604-022-05284-8

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  • DOI: https://doi.org/10.1007/s00604-022-05284-8

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