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Glucose determination using amperometric non-enzymatic sensor based on electroactive poly(caffeic acid)@MWCNT decorated with CuO nanoparticles

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Abstract

A novel non-enzymatic glucose sensor based on poly(caffeic acid)@multi-walled carbon nanotubes decorated with CuO nanoparticles (PCA@MWCNT-CuO) was developed. The described approach involves the complexation/accumulation of Cu(II) on PCA@MWCNT followed by electrochemical CuO deposition in an alkaline electrolyte. The morphology and surface characteristics of the nanomaterial were determined by transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS), atomic force microscopy (AFM), Raman spectroscopy, and inductively coupled plasma mass spectrometry (ICP-MS). A hybrid-support sensor device was then developed to assess the glucose concentration in different solutions. The sensitivity of the electrode is 2412 μA mM−1 cm−2. The electrode exhibited a broad linear range of 2 µM to 9 mM and a low limit of detection (LOD) of 0.43 µM (relative standard deviation, RSD = 2.3%) at + 0.45 V vs Ag/AgCl. The excellent properties obtained for glucose detection were most likely due to the synergistic effect of the combination of individual components: poly(caffeic acid), MWCNTs, and CuO. Good accuracy and high precision were demonstrated for quantifying glucose concentrations in human serum and blood samples (the recovery ranged from 95.0 to 99.5%). The GC/PCA@MWCNT-CuO sensor represents a novel, simple, and low-cost approach to the fabrication of devices for amperometric sensing of glucose.

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Funding

This work was financed and prepared as part of a research project supported by the National Science Centre Poland, no. 2017/27/B/ST8/01506.

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Authors and Affiliations

  1. Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60965, Poznan, Poland

    Maria Kuznowicz, Artur Jędrzak & Teofil Jesionowski

  2. Faculty of Chemical Technology, Institute of Chemistry and Technical Electrochemistry, Poznan University of Technology, Berdychowo 4, 60965, Poznan, Poland

    Tomasz Rębiś

  3. NanoBioMedical Centre, Adam Mickiewicz University, Wszechnicy Piastowskiej 3, 61614, Poznan, Poland

    Artur Jędrzak & Grzegorz Nowaczyk

  4. Faculty of Materials Engineering and Technical Physics, Institute of Materials Research and Quantum Engineering, Poznan University of Technology, Piotrowo 3, 60965, Poznan, Poland

    Mirosław Szybowicz

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  1. Maria Kuznowicz
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  2. Tomasz Rębiś
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  3. Artur Jędrzak
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  4. Grzegorz Nowaczyk
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  5. Mirosław Szybowicz
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  6. Teofil Jesionowski
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Correspondence to Tomasz Rębiś or Teofil Jesionowski.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors. Informed consent was obtained from all individual participants included in the study.

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Kuznowicz, M., Rębiś, T., Jędrzak, A. et al. Glucose determination using amperometric non-enzymatic sensor based on electroactive poly(caffeic acid)@MWCNT decorated with CuO nanoparticles. Microchim Acta 189, 159 (2022). https://doi.org/10.1007/s00604-022-05256-y

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