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Heparin-enhanced peroxidase-like activity of iron-cobalt oxide nanosheets for sensitive colorimetric detection of trypsin

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Abstract

Iron-cobalt oxide nanosheets (FeCo-ONSs) were proved to have intrinsic peroxidase-like activity. Additionally, the peroxidase-like activity of FeCo-ONSs toward the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) was dramatically enhanced after heparin addition due to the stronger affinity toward TMB. Protamine combines with heparin, so the promotion of peroxidase-like activity of FeCo-ONSs with heparin was suppressed. With the addition of trypsin, protamine was hydrolyzed and the enhancement effect of catalytic activity of FeCo-ONSs was recovered. Based on above process, a sensitive colorimetric platform for trypsin activity determination was constructed through measuring the absorbance of produced oxTMB at 652 nm, providing a linear detection range of 5 to 500 ng/mL and a low detection limit of 2.8 ng/mL. The method was applied to trypsin determination in real samples (human urine sample and multienzyme tablet sample) with satisfactory results, illustrating the potential application of this biosensor.

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Acknowledgements

This work is supported by the National Natural Science Foundation of China (No. 21775052 and No. 21575048) and the Science and Technology Development project of Jilin province, China (No. 20180414013GH).

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Authors and Affiliations

  1. Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China

    Chenyu Zhou, Junyang Chen & Xingguang Su

  2. College of Medical Engineering, Jining Medical University, Jining, 272067, People’s Republic of China

    Guannan Wang

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Correspondence to Guannan Wang or Xingguang Su.

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Zhou, C., Chen, J., Wang, G. et al. Heparin-enhanced peroxidase-like activity of iron-cobalt oxide nanosheets for sensitive colorimetric detection of trypsin. Microchim Acta 189, 135 (2022). https://doi.org/10.1007/s00604-022-05227-3

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