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Novel electrochemical sensor modified with molecularly imprinted polymers for determination of enrofloxacin in marine environment

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Abstract

Molecularly imprinted polymers were synthesized by gel–sol method with multi-walled carbon nanotubes as support and enrofloxacin as a template and further modified on the surface of glassy carbon electrode to construct a molecularly imprinted electrochemical sensor. The performance of the imprinted electrochemical sensor was thoroughly investigated by using cyclic voltammetry and differential pulse voltammetry. The influence of imprinted polymers amount, electrolyte pH, and incubation time on the sensor performance was investigated for the detection of enrofloxacin. Under the optimal experimental conditions in a three-electrode system with the modified electrode as the working electrode the differential pulse voltammetry response current of the sensor had a good linear relationship at 0.2 V (vs. saturated calomel reference electrode) with the enrofloxacin concentration within 2.8 pM–28 μM and the limit of detection of the method was 0.9 pM. The competitive interference experiment showed that the imprinted electrochemical sensor could selectively recognize enrofloxacin. The method was applied to analyze spiked natural seawater, fish, and shrimp samples. The recovery was 96.4%–102%, and RSD was less than 4.3% (n = 3), indicating that the proposed imprinted electrochemical sensor was suitable for the determination of trace enrofloxacin in marine environment samples.

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Funding

This work was supported by the National Key R&D Program of China [grant number 2019YFC140027-02, 2016YFC1402101], the National Natural Science Foundation of China [grant number 41876078], and The Postdoctoral Innovative Talents Support Program of Shandong Province [grant number SDBX2020015].

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Authors and Affiliations

  1. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China

    Jianlei Chen, Liju Tan & Jiangtao Wang

  2. Marine Fishery Environment and Bioremediation Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Jianlei Chen, Keming Qu & Zhengguo Cui

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  1. Jianlei Chen
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  2. Liju Tan
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  3. Keming Qu
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Correspondence to Keming Qu or Jiangtao Wang.

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Chen, J., Tan, L., Qu, K. et al. Novel electrochemical sensor modified with molecularly imprinted polymers for determination of enrofloxacin in marine environment. Microchim Acta 189, 95 (2022). https://doi.org/10.1007/s00604-022-05205-9

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