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Synthesis of a cerium-based nanomaterial with superior oxidase-like activity for colorimetric determination of glutathione in food samples

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Abstract

Enzyme-like nanomaterials have received significant attention for their high stability and low cost. However, most nanomaterials require complicated synthesis processes, limiting the range of their potential applications. In this study, a novel cerium-based nanomaterial was fabricated in a facile manner from a mixture of dipicolinic acid (DPA), guanosine 5′-monophosphate (GMP), and cerium acetate under ambient conditions. The obtained nanomaterial, designated as DPA–Ce–GMP, exhibited superior oxidase-like activity owing to the mixed valence (Ce3+/Ce4+) of cerium ions. DPA–Ce–GMP efficiently catalyzed the oxidation of 3,3,5,5-tetramethylbenzidine (TMB), achieving a color reaction without requiring hydrogen peroxide. Thus, DPA–Ce–GMP was incorporated into a simple, rapid, and sensitive colorimetric sensor for glutathione (GSH) detection. Within this sensor, TMB oxidation is inhibited by the reducibility of GSH. The sensor exhibits a linear response over two concentration ranges (0.05–10 and 10–40 μM), and its detection limit is 17.1 nM (3σ/slope). The proposed sensor was successfully applied to GSH quantification in food samples. The developed sensor provides an efficient biomimic oxidase for GSH detection in real samples.

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Facile approach to prepare cerium-based nanomaterial with superior oxidase-like activity for colorimetric detection of glutathione in food samples

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Funding

This work was supported by the National Natural Science Foundation of China (No. 32172298).

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Authors and Affiliations

  1. Henan Engineering Technology Research Center of Food Processing and Circulation Safety Control, College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, Henan, China

    Tianlin Wang, Mengying Liang, Lianjun Song, Tiange Li, Xiya Zhang, Ning Li & Xianqing Huang

  2. Henan Technology Innovation Center of Meat Processing and Research, College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, Henan, China

    Tianlin Wang, Mengying Liang, Lianjun Song, Tiange Li, Xiya Zhang, Ning Li & Xianqing Huang

  3. College of Food Science, Sichuan Agricultural University, Yaan, 625000, Sichuan, China

    Yuwen Hu

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  1. Tianlin Wang
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Correspondence to Xianqing Huang.

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Wang, T., Hu, Y., Liang, M. et al. Synthesis of a cerium-based nanomaterial with superior oxidase-like activity for colorimetric determination of glutathione in food samples. Microchim Acta 189, 132 (2022). https://doi.org/10.1007/s00604-022-05197-6

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