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A novel colorimetric aptasensor for sensitive tetracycline detection based on the peroxidase-like activity of Fe3O4@Cu nanoparticles and “sandwich” oligonucleotide hybridization

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Abstract

A novel colorimetric aptasensor has been developed for highly sensitive tetracycline (TC) detection based on the peroxidase-like activity of Fe3O4@Cu nanoparticles and “sandwich” oligonucleotide hybridization. The Fe3O4@Cu nanoparticles with high peroxidase-like activity were successfully synthesized under mild conditions. Then, a “sandwich” oligonucleotide hybridization probe (a short amino-modified complementary sequence of a portion of the TC aptamer (cDNA1), TC aptamers, and a long complementary to 5′ terminal TC aptamer sequence (cDNA2)) was created in 96-wells plates via DNA hybridization in the absence of TC from the detection system. The unique “sandwich” oligonucleotide hybridization probe adsorbed large numbers of Fe3O4@Cu nanozymes while further enhancing its peroxidase-like activity. Based on the 3,3′,5,5′-tetramethylbenzidine (TMB)-hydrogen peroxide (H2O2) reporting system, the blue color of the solution decreased linearly with the increase of TC concentration, ranging from 10−3 to 103 μg/L with an ultralow limit of detection (LOD) of 0.91 ng/L (2 pM). The proposed method was successfully applied to detect TC in spiked milk samples, with recoveries of 81.8 to 112%, demonstrating the excellent potential for highly sensitive TC detection in milk.

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Funding

This work was financially supported by the Chunhui Program Research Project of the Chinese Ministry of Education (192624), the National Nature Science Foundation of China (31801647), and the Sichuan Science and Technology Program (2020YFN0041, 2020YFN0151, 2020YFN0153).

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Authors and Affiliations

  1. School of Food and Bioengineering, Xihua University, Chengdu, 610039, China

    Lijun Wang, Hong Zhou, Xue Wu, Yanfei Song, Yukun Huang, Xiao Yang & Xianggui Chen

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  1. Lijun Wang
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  2. Hong Zhou
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  3. Xue Wu
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  4. Yanfei Song
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  5. Yukun Huang
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  6. Xiao Yang
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  7. Xianggui Chen
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Correspondence to Yukun Huang or Xianggui Chen.

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Wang, L., Zhou, H., Wu, X. et al. A novel colorimetric aptasensor for sensitive tetracycline detection based on the peroxidase-like activity of Fe3O4@Cu nanoparticles and “sandwich” oligonucleotide hybridization. Microchim Acta 189, 86 (2022). https://doi.org/10.1007/s00604-022-05195-8

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  • DOI: https://doi.org/10.1007/s00604-022-05195-8

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