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Photothermal card reader assay using the commercial colloidal gold test strip for the rapid quantitative detection of food hazards

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Abstract

The simple and rapid commercial colloidal gold test strip can only be used for qualitative or semi-quantitative detection, accompanied by weak detectability and false negative experimental results. Herein, a photothermal test strip assay which combined test strip with a portable photothermal card reader was established to achieve quantitative detection with excellent detectability. According to the photothermal effect produced by gold nanoparticles (GNPs) captured on the test line, the signal could be recorded by the reader. Thirteen food hazards including veterinary drug residues and pesticide residues were tested; the photothermal detectability in actual samples were about 23 (methyl parathion), 7 (enrofloxacin), 6 (sarafloxacin), 8 (sulfadiazine), 12 (sulfamethazine), 7 (paraquat), 6 (malachite green), 11 (amantadine), 13 (nitrofurazone), 6 (diethylstilbestrol), 12 (estriol), 21 (estrone), and 26 (17β-estradiol) times better than the visual detectability. Our results demonstrated that the photothermal test strip assay could be used for sensitive, rapid, and quantitative detection of residues of food hazards.

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Funding

This work was supported by the National Natural Science Foundation of China (31871874 and 32002098) and the Natural Science Foundation of Shandong Province (ZR202010290039).

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Authors and Affiliations

  1. College of Food Science and Engineering, Shandong Agricultural University, Tai’an, 271018, People’s Republic of China

    Ying Wang & Zhixiang Xu

  2. Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong, Normal University, College of Life Sciences, Shandong Normal University, Jinan, 250014, People’s Republic of China

    Tian Wang, Minglu Wang & Hongyan Zhang

  3. Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes, Shandong Institute for Food and Drug Control, Jinan, 250101, People’s Republic of China

    Jun Wang

Authors
  1. Ying Wang
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  2. Tian Wang
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  3. Minglu Wang
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  4. Jun Wang
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  5. Zhixiang Xu
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  6. Hongyan Zhang
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Contributions

Ying Wang: synthesis, investigation, writing–original draft. Tian Wang: investigation and results. Minglu Wang: validation, writing–review and editing. Shuyuan Du: data curation. Jun Wang: reproducibility. Zhixiang Xu and Hongyan Zhang: writing–review and editing, supervision.

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Correspondence to Zhixiang Xu or Hongyan Zhang.

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Wang, Y., Wang, T., Wang, M. et al. Photothermal card reader assay using the commercial colloidal gold test strip for the rapid quantitative detection of food hazards. Microchim Acta 189, 112 (2022). https://doi.org/10.1007/s00604-022-05193-w

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  • DOI: https://doi.org/10.1007/s00604-022-05193-w

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