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Electrochemical sensing of Staphylococcus aureus based on conductive anti-fouling interface

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Abstract

A system for the rapid and ultra-sensitive detection of Staphylococcus aureus (S. aureus), a prevalent foodborne pathogen is introduced. Limitations of typical electrochemical sensing, often subjected to interference from non-specific protein adsorption are addressed. A dual-aptamer-based sandwich immunobiosensor is shown for its benefits regarding specificity and anti-fouling capacity, endowed by a sulfonated polyaniline layer combined with signal amplification via highly conductive gold nanoparticles. EIS spectra (Nyquist plots) were recorded at pH 7.4 PBS containing 5 mM Fe(CN)63−/Fe(CN)64−, in order to verify the possibility of the electrochemical sensing for detection of S. aureus. Results demonstrated that the constructed immunobiosensor presents an extended detection range (1 × 101 to 1 × 105 CFU/mL) and detection limit as low as 2 CFU/mL. The resistance values of the immunobiosensor developed  maintain at a stable value during 2 weeks.  Besides, the specificity of the system is highlighted by testing raw milk, and the results of which demonstrate the excellent prospects of the system for monitoring foodborne pathogens.

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Acknowledgements

This work was supported by the University Natural Science Research Project of Jiangsu Province (19KJB430026), the Foundation (GZKF202015) of State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Nanjing Forestry University 2018 Scientific Research Start-up Funds (163105042), and the financial support from China Scholarship Council (CSC).

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Authors and Affiliations

  1. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, China

    Huanli Gao, Tingting Xu, Jiahuan Zhou & Hongqi Dai

  2. Bioproducts Institute, Department of Chemical and Biological Engineering, Department of Chemistry and Department of Wood Science, The University of British Columbia, 2360 East Mall, Vancouver, BC, V6T 1Z3, Canada

    Tingting Xu & Orlando J. Rojas

  3. State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China

    Tingting Xu, Ming He & Xingxiang Ji

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  1. Huanli Gao
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  2. Tingting Xu
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  3. Jiahuan Zhou
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  4. Orlando J. Rojas
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  5. Ming He
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  7. Hongqi Dai
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Correspondence to Tingting Xu or Hongqi Dai.

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Gao, H., Xu, T., Zhou, J. et al. Electrochemical sensing of Staphylococcus aureus based on conductive anti-fouling interface. Microchim Acta 189, 97 (2022). https://doi.org/10.1007/s00604-022-05190-z

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  • DOI: https://doi.org/10.1007/s00604-022-05190-z

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