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New trends in enzyme-free electrochemical sensing of ROS/RNS. Application to live cell analysis

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A Correction to this article was published on 06 August 2022

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Abstract

The ubiquity and importance of ROS and RNS in cellular signaling, disease development, and death give rise to an outstanding interest in their detection and quantification. Among the analytical techniques available, electrochemical sensors stand out for the detection of ROS/RNS due to their high sensitivity and inherent miniaturization which allows the in situ and real-time detection together with a tunable selectivity due to the different electrochemical behavior of ROS/RNS. Nanomaterial-based enzyme-free electrochemical sensors possess improved sensitivity, selectivity, stability, and unique catalytic activities. In addition, their integration in nanoelectrodes, lab-on-chips, microfluidic systems, and stretchable electrodes allow the determination of ROS/RNS in individual cells, cell organelles, or cell populations, under different experimental conditions hardly accessible using classical detection methods.

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Acknowledgements

D.R. acknowledges European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement N°713714 and co-funding of University of Teramo and Abruzzo region.

J.F.H-R. and A.E. acknowledge financial support from the TRANSNANOAVANSENS program (S2018-NMT-4349) from the Community of Madrid. AE also acknowledges Spanish Ministry of Economy, Industry and Competitiveness (CTQ2017-86441-C2-1-R). J.F.H-R also acknowledges the FPI fellowship received from the University of Alcalá.

F.D.P. and D.C. acknowledge the Ministry of Education, University and Research (MIUR) and European Social Fund (ESF) for the PON R&I 2014–2020 program, action 1.2 “AIM: Attraction and International Mobility” (AIM1894039-3).

D.C. acknowledges the PRIN 2017 ACTUaL project of the Italian Ministry of Education, University and Research (MIUR).

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Authors and Affiliations

  1. Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus “Aurelio Saliceti” via R. Balzarini 1, 64100, Teramo, Italy

    Daniel Rojas, Flavio Della Pelle & Dario Compagnone

  2. Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Sciences, University of Alcalá, 28871, Alcalá de Henares, Madrid, Spain

    Daniel Rojas, Juan F. Hernández-Rodríguez & Alberto Escarpa

  3. Chemical Research Institute Andres M. del Rio, University of Alcalá, 28871, Madrid, Spain

    Alberto Escarpa

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  1. Daniel Rojas
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  2. Juan F. Hernández-Rodríguez
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  3. Flavio Della Pelle
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  4. Alberto Escarpa
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  5. Dario Compagnone
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Correspondence to Alberto Escarpa or Dario Compagnone.

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The original online version of this article was revised: In this article, Figs. 1, 3, 4 must be updated so caption and image are within the page. Given here are the corrected figures.

Glossary

17-oxo-DHA:

17-Oxo-docosahexaenoic acid

A431:

Model human cell line epidermoid carcinoma

A549:

Adenocarcinomic human alveolar basal epithelial cells

AA:

Ascorbic acid

ALA:

Alpha lipoic acid

AuNFs:

Gold nanoflowers

AuNTs:

Gold nanotubes

AuNWs:

Gold nanowires

Aβ:

Amyloid β protein

CaCo-2:

Immortalized cell line of human colorectal adenocarcinoma cells

CB:

Carbon black

CHAPS:

3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate

CNTs:

Carbon nanotubes

CTS:

Chitosan

DLTA:

DL-thioctic acid

DPV:

Differential pulsed voltammetry

fMLP:

N-formylmethionyl-leucyl-phenylalanine peptide

GCE:

Glassy carbon electrode

GNs:

Graphene nanosheets

GSH:

Glutathione

GSH:

Reduced glutathione

H9C2:

Cell model used as an alternative for cardiomyocytes

HAT:

1-Hexanethiol

HEBEC:

Human bronchial epithelial cells

HEK-293:

Normal fetal human embryonic kidney cells

HepG2:

Human liver cancer cell line

DU145:

Human prostate cancer cells

HUVEC:

Human umbilical vein endothelial cells. Are primary human cells isolated from the vein of the umbilical cord

IFN-γ:

Interferon-γ

ITO:

Indium tin oxide

L-Arg:

L-Arginine

L-NAME:

N-nitroarginine methyl ester

LNCaP:

Cell line derived from a metastatic lymph node lesion of human prostate cancer

LPS:

Lipopolysaccharide

MCF-7:

Breast cancer cell line isolated

MDA-MB-231:

Human breast adenocarcinoma cells. Cell line isolated from the breast tissue of an adenocarcinoma patient

MEA:

Microelectrode array

MOF:

Metal-organic framework

MP:

4-Methoxy phenol

MPNS:

Microporous polymeric nanospheres

MWCNTs:

Multiwalled carbon nanotubes

NAC:

N-acetyl-L-cysteine

NCS:

N-doped carbon spheres

NGS:

N-doped graphene nanosheets

OFET:

Organic field-effect transistor

PB:

Prussian blue

PC12:

Cell line derived from a pheochromocytoma of the rat adrenal medulla

PDA:

Polydopamine

PDMS:

Polydimethylsiloxane

PET:

Polyethylene terephthalate

PHT:

Poly(3-hexylthiophene)

PLLA-PTMC:

Copolymer of poly(L-lactic acid) and polytrimethylene carbonate

PMA:

Phorbol 12-myristate 13-acetate

Raw 264.7:

Macrophage-like cells, originating from Abelson leukemia virus transformed cell line derived from BALB/c mice

rGO:

Reduced graphene oxide

RNS:

Reactive nitrogen species

ROS:

Reactive oxygen species

SAM:

Self-assembled monolayer

SECM:

Scanning electrochemical microscopy

SH-5Y5Y:

Neuroblastoma cell line. It serves as a model for neurodegenerative disorders

SK-OV-3:

Ovarian human cancer–derived cell line

SPCE:

Screen-printed carbon electrode

THP-1:

Immortalized monocyte-like cell line, derived from the peripheral blood of a childhood case of acute monocytic leukemia

U87:

Cell line derived from human malignant gliomas

UA:

Uric acid

UME:

Ultramicroelectrodes

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Rojas, D., Hernández-Rodríguez, J.F., Della Pelle, F. et al. New trends in enzyme-free electrochemical sensing of ROS/RNS. Application to live cell analysis. Microchim Acta 189, 102 (2022). https://doi.org/10.1007/s00604-022-05185-w

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