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Phage display-derived immunorecognition elements LSPR nanobiosensor for peptide hormone glycine-extended gastrin 17 detection

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Abstract

The current study intended to evaluate two types of biorecognition element (BRE), namely recombinant antibody fragments and M13 bacteriophage-displayed antibody fragments, where protein L and electrostatic interactions were used to respectively conjugated antibodies and bacteriophages on AuNPs. The functionalization process was examined by DLS to monitor the changes in the size and zeta potential. The formation of the BRE-G17-Gly immunological complexes was manifested by aggregation (confirmed by FE-SEM) and color change from red to dark blue visible to the naked eye. Local refractive index variations of functionalized AuNPs were monitored by a UV − vis spectrophotometer, showing increasing size and decreasing zeta potential in all stages. The calibration plot was developed in the concentration range 1–5 µg/mL and the limit of detection (LOD) was 1 µg/mL. The LSRP nanobiosensor in combination with the phage-based BRE was an affordable and simple approach, as it was able to eliminate the time-consuming and costly step of extracting antibodies. Contrary to the traditional immunoassays, this method does not require additional amplification, e.g., enzymatic, to read the result. The proposed LSPR nanobiosensor model can be adapted to detect a wide range of pathogens, viruses, and biomarkers in the shortest possible time.

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Data sharing is not applicable to this article as all data created or analyzed in this study are detailed in the results section in the manuscript.

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Acknowledgements

This project is part of an M.Sc. thesis conducted at Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, and was supported by the Tabriz University of Medical Sciences.

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Authors and Affiliations

  1. Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran

    Zahra Fattahi & Ahmad Yari Khosroushahi

  2. Research Center for Pharmaceutical Nanotechnology, Research and Development Complex, Tabriz University of Medical Sciences, Daneshgah Street, Tabriz, Iran

    Mohammad Reza Tohidkia

  3. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Ahmad Yari Khosroushahi

Authors
  1. Zahra Fattahi
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  2. Mohammad Reza Tohidkia
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  3. Ahmad Yari Khosroushahi
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Correspondence to Mohammad Reza Tohidkia or Ahmad Yari Khosroushahi.

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Fattahi, Z., Tohidkia, M.R. & Yari Khosroushahi, A. Phage display-derived immunorecognition elements LSPR nanobiosensor for peptide hormone glycine-extended gastrin 17 detection. Microchim Acta 189, 48 (2022). https://doi.org/10.1007/s00604-021-05159-4

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