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An immunoassay based on nanomotor-assisted electrochemical response for the detection of immunoglobulin

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Abstract

An immunoassay strategy has been developed based on nanomotor-assisted electrochemical measurements for simple and sensitive detection of immunoglobulin (IgG). The self-propelled Fe3O4@SiO2/Pt nanomotors were designed to label primary antibodies IgG (nanomotor-label) for the “on-the-fly” binding of the immune-protein. The core shell Au@Ag nanocubes (Au@Ag NCs) were used as labels of secondary antibodies (Au@Ag NCs-Ab2) to amplify electrochemical signal related to antigen concentration derived from the oxidation of Ag. The self-propelled nanomotors autonomously move in the solution to cruise and capture IgG and Au@Ag NCs-Ab2, resulting in the self-assembly of sandwich immune-complex. Finally, the immune-complex with magnetism can be transferred and modified on the electrode for the detection of IgG via differential pulse voltammetry. The self-propelled motion of the nanomotor-label obviates common procedures for the self-assembly of sandwich immunosensors to achieve satisfactory analysis results. With advantages of automation and miniaturization, the strategy based on self-propelled nanomotor-labels explores an effective method for the simple and sensitive detection of immune-protein in biosensing.

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Funding

This work is supported by the National Natural Science Foundation of China (21905303) and the Fundamental Research Funds for the Central Universities (2020ZDPY0213).

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Authors and Affiliations

  1. School of Chemical Engineering & Technology, China University of Mining and Technology, Xuzhou, Jiangsu, 221116, People’s Republic of China

    Enhui Ma, Ke Wang & Hong Wang

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  1. Enhui Ma
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  2. Ke Wang
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  3. Hong Wang
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Correspondence to Hong Wang.

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Ma, E., Wang, K. & Wang, H. An immunoassay based on nanomotor-assisted electrochemical response for the detection of immunoglobulin. Microchim Acta 189, 47 (2022). https://doi.org/10.1007/s00604-021-05158-5

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  • DOI: https://doi.org/10.1007/s00604-021-05158-5

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