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A molecularly imprinted electrochemical biosensor based on hierarchical Ti2Nb10O29 (TNO) for glucose detection

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Abstract

A novel molecularly imprinted electrochemical biosensor for glucose detection is reported based on a hierarchical N-rich carbon conductive-coated TNO structure (TNO@NC). Firstly, TNO@NC was fabricated by a novel polypyrrole-chemical vapor deposition (PPy-CVD) method with minimal waste generation. Afterward, the electrode modification with TNO@NC was performed by dropping TNO@NC particles on glassy carbon electrode surfaces by infrared heat lamp. Finally, the glucose-imprinted electrochemical biosensor was developed in presence of 75.0 mM pyrrole and 25.0 mM glucose in a potential range from + 0.20 to + 1.20 V versus Ag/AgCl via cyclic voltammetry (CV). The physicochemical and electrochemical characterizations of the fabricated molecularly imprinted biosensor was conducted by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) method, X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS), and CV techniques. The findings demonstrated that selective, sensitive, and stable electrochemical signals were proportional to different glucose concentrations, and the sensitivity of molecularly imprinted electrochemical biosensor for glucose detection was estimated to be 18.93 μA μM−1 cm−2 (R2 = 0.99) at + 0.30 V with the limit of detection (LOD) of 1.0 × 10−6 M. Hence, it can be speculated that the fabricated glucose-imprinted biosensor may be used in a multitude of areas, including public health and food quality.

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Acknowledgements

Mehmet Lütfi Yola would like to thank Turkish Academy of Sciences for their invaluable support in respect to Young Scientists Award Program, TÜBA-GEBIP (2019).

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Authors and Affiliations

  1. Department of Electricity and Energy, Vocational School of Technical Sciences, Akdeniz University, Antalya, Turkey

    Ceren Karaman

  2. Department of Medical Imaging Techniques, Vocational School of Health Services, Akdeniz University, Antalya, Turkey

    Onur Karaman

  3. Department of Chemical Engineering, Faculty of Engineering, Pamukkale University, Denizli, Turkey

    Necip Atar

  4. Department of Nutrition and Dietetics, Faculty of Health Sciences, Hasan Kalyoncu University, Gaziantep, Turkey

    Mehmet Lütfi Yola

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  1. Ceren Karaman
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Karaman, C., Karaman, O., Atar, N. et al. A molecularly imprinted electrochemical biosensor based on hierarchical Ti2Nb10O29 (TNO) for glucose detection. Microchim Acta 189, 24 (2022). https://doi.org/10.1007/s00604-021-05128-x

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