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Capillary-based fluorescence microsensor with polyoxometalates as nanozyme for rapid and ultrasensitive detection of artemisinin

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Abstract

A novel capillary-based fluorescence microsensor for artemisinin was developed with functional polyoxometalates (POMs) as nanozyme by a layer-by-layer self-assembly strategy. Vanadomolybdophosphoric heteropoly acid (H5PMo10V2O40, PMoV2) and tungstophosphoric heteropoly acid (Na5PW11O39Cu, PW11Cu) with high peroxidase-like activity were synthesized and immobilized on capillary to catalyze artemisinin/thiamine reaction and generate the amplified fluorescence signal. The wide linear range up to 13.0 μM with the low limit of detection of 0.03 μM (S/N = 3) was achieved for the determination of artemisinin by using the proposed POMs-microsensor. The method has been successfully used to detect artemisinin in human plasma and antimalarial drugs with satisfactory accuracy. This work developed a novel capillary fluorescence microsensor with functional POMs as nanozyme, which can serve as a promising candidate in fluorescence microanalysis.

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Acknowledgements

L. Yang would also like to thank the support from Jilin Provincial Department of Education and Jilin Provincial Key Laboratory of Micro-Nano Functional Materials (Northeast Normal University).

Funding

This work is supported by the National Natural Science Foundation of China (Grant nos. 22074014) and the Natural Science Foundation of Jilin Province, China (Grant No. 20200404153YY).

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Authors and Affiliations

  1. Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province, 130024, China

    Jinlan Yang, Yulan Gao & Li Yang

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  1. Jinlan Yang
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  2. Yulan Gao
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  3. Li Yang
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Correspondence to Li Yang.

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Jinlan Yang and Yulan Gao are contributed equally

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Yang, J., Gao, Y. & Yang, L. Capillary-based fluorescence microsensor with polyoxometalates as nanozyme for rapid and ultrasensitive detection of artemisinin. Microchim Acta 189, 40 (2022). https://doi.org/10.1007/s00604-021-05126-z

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