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Mesoporous nanomaterial-assisted hydrogel double network composite for mixed-mode liquid chromatography

Abstract

By introducing functional groups such as quaternary amine groups, sulfonic acid groups, triazine groups, and other mespore nanomaterials into the hydrogel, better separation effect of some organic framework materials has been obtained. Due to a reasonable design and preparation strategy, the hydrogel composite-modified silica can be used in the selective separation of various analytes such as pesticides, alkylbenzenes, polycyclic aromatic hydrocarbons, nucleosides/bases, benzoic acids, antibiotics, and carbohydrates. Through the exploration of chromatographic retention behavior, it is proved that the column can be used in mixed-mode liquid chromatography. The intra-day relative standard deviation for retention time of this new stationary phase is 0.12–0.16% (n = 10), and the inter-day relative standard deviation is less than 0.39% (n = 5). This new stationary phase can also be used for separation in complex samples. The limit of detection (LOD) for chlorotoluron in farm irrigation water is 0.21 µg/L and the linear range is 2–250 µg/L. After optimizing the chromatographic conditions, the highest efficiency of the hydrogel column in RPLC and HILIC modes has reached 32,400 plates/m (chlorobenzuron) and 41,300 plates/m (galactose). This new type of hydrogel composite is a porous network material with flexible functional design and simple preparation method and its application has been expanded in liquid chromatography separation successfully.

Graphical abstract

The hydrogel composed of triallyl cyanate cross-linking agent and 3-(2-(methacryloyloxy) ethyl) dimethylamine) propane-1-sulfonate (SBMA) monomer which were co-modified on the surface of mesoporous silica with MOF-919 for separation in mixed-mode liquid chromatography.

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Funding

This work was supported by the National Natural Science Foundation of China (Nos. 21575149, 21575148).

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Authors and Affiliations

Authors

Contributions

Fangbin Fan: conceptualization, method, investigation, data curation, and writing—original draft.

Xiaofeng Lu: formal analysis.

Shuai Wang: software and resources.

Licheng Wang: validation and formal analysis.

Xiaojing Liang: writing—review and editing.

Yong Guo: funding acquisition and writing—review and editing.

Corresponding authors

Correspondence to Xiaojing Liang or Yong Guo.

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The authors declare no competing interests.

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Fan, F., Lu, X., Wang, S. et al. Mesoporous nanomaterial-assisted hydrogel double network composite for mixed-mode liquid chromatography. Microchim Acta 188, 433 (2021). https://doi.org/10.1007/s00604-021-05094-4

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  • DOI: https://doi.org/10.1007/s00604-021-05094-4

Keywords

  • Functional design
  • Liquid chromatography
  • Mixed-mode stationary phase
  • Hydrogel composite
  • MOF-919
  • Porous network