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Wearable healthcare smart electrochemical biosensors based on co-assembled prussian blue—graphene film for glucose sensing

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Abstract

Wearable film-based smart biosensors have been developed for real-time biomolecules detection. Particularly, interfacial co-assembly of reduced graphene oxide-prussian blue (PB-RGO) film through electrostatic interaction has been systematically studied by controllable pH values, achieving optimal PB-RGO nanofilms at oil/water (O/W) phase interface driven by minimization of interfacial free energy for wearable biosensors. As a result, as-prepared wearable biosensors of PB-RGO film could be easily woven into fabrics, exhibiting excellent glucose sensing performance in amperometric detection with a sensitivity of 27.78 µA mM−1 cm−2 and a detection limit of 7.94 μM, as well as impressive mechanical robustness of continuously undergoing thousands of bending or twist. Moreover, integrated wearable smartsensing system could realize remotely real-time detection of biomarkers in actual samples of beverages or human sweat via cellphones. Prospectively, interfacial co-assembly engineering driven by pH-induced electrostatic interaction would provide a simple and efficient approach for acquiring functional graphene composites films, and further fabricate wearable smartsensing devices in health monitoring fields.

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Funding

This work was supported by Dalian Science and Technology Bureau, China [Grant No. 2019J12SN54], National Natural Science Foundation of China [Grant No. 22074010], and Zhang Dayu School of Chemistry, Dalian University of Technology, China.

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Authors and Affiliations

  1. Zhang Dayu School of Chemistry, State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, Liaoning, 116024, China

    Junlin Ma, Yuhang Du, Yu Jiang, Liuxue Shen, Hongting Ma, Fengjuan Lv, Zewei Cui, Lei Shi & Nan Zhu

  2. School of Chemical Engineering, Dalian University of Technology, Dalian , 116024, Liaoning, China

    Yuzhen Pan

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  1. Junlin Ma
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Contributions

N.Z. conceived the idea and project. J.M. performed the experiment and data analysis. Y.D., Y.J., L.S., H.M., F.L., Z.C., Y.P., L.S. performed partial experiment and data analysis. J.M. and N.Z. jointly wrote the paper. All the authors discussed the results and commented on the manuscript.

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Correspondence to Nan Zhu.

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Ma, J., Du, Y., Jiang, Y. et al. Wearable healthcare smart electrochemical biosensors based on co-assembled prussian blue—graphene film for glucose sensing. Microchim Acta 189, 46 (2022). https://doi.org/10.1007/s00604-021-05087-3

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