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Cavitas electrochemical sensors for the direct determination of salivary thiocyanate levels

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Abstract

Noninvasive diagnosis using salivary samples to detect thiocyanate provides vital information on individual health. This article demonstrates the first example of a wearable sensing device to noninvasively assess thiocyanate levels. The customized screen-printed electrode system is integrated into a form of a mouthguard squarewave-voltammetric sensor toward the convenient and fast detection of the salivary biomarker within 15 s. The sensor with a protective film to mitigate the effect of biofouling offers high sensitivity and selectivity toward the detection of thiocyanate ions. Partial least square regression is applied to analyze the high-order squarewave-voltammetric data over the applied potential range of 0–1.75 V vs Ag/AgCl and quantify the thiocyanate concentration in a complex matrix. The mouthguard sensor operating under physiological conditions can monitor a wide range of thiocyanate (up to 11 mM) with a low detection limit of 30 µM. The demonstration introduces a unique approach, that obviates the requirement for blood sampling, to study thiocyanate levels of healthy people, cigarette smokers, or people with other health conditions. It is envisioned that the new cavitas device possesses a substantial promise for diverse biomedical diagnosis applications.

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Acknowledgements

We would like to thank to Talent Management Project of Prince of Songkla University. We also gratefully acknowledge the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research, and Innovation (MHESI).

Funding

This project was supported by the Faculty of Science Research Fund 2021 (Contract Number: 264003), Prince of Songkla University, Hat Yai, Thailand.

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Authors and Affiliations

  1. Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand

    Rachanon Sangsawang, Chongdee Buranachai, Panote Thavarungkul, Proespichaya Kanatharana & Itthipon Jeerapan

  2. Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand

    Chongdee Buranachai, Panote Thavarungkul, Proespichaya Kanatharana & Itthipon Jeerapan

  3. Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand

    Chongdee Buranachai, Panote Thavarungkul, Proespichaya Kanatharana & Itthipon Jeerapan

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  1. Rachanon Sangsawang
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Correspondence to Itthipon Jeerapan.

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Sangsawang, R., Buranachai, C., Thavarungkul, P. et al. Cavitas electrochemical sensors for the direct determination of salivary thiocyanate levels. Microchim Acta 188, 415 (2021). https://doi.org/10.1007/s00604-021-05067-7

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