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Graphene oxide–rhodamine nanocomposite for picomolar detection of chromium(III) by fluorimetry and its biofilm inhibition

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Abstract

Graphene oxide–rhodamine B hydrazide (GO-RhB) nanocomposite was prepared by a simple chemical method and characterized by various spectroscopic and analytical techniques. GO-RhB nanocomposite potentially detects Cr3+ ion (excitation/emission = 550 nm/572 nm) via fluorescence turn “on–off” approach. This composite showed high binding affinity (106 M−1) with Cr3+ and a+ limit of detection (LOD) down to picomolar concentration (LOD = 85.6 pM). As far as we know, this is the first report for the sensing of Cr3+ ion at picomolar concentration. GO-RhB selectively senses Cr3+ ion without any interference of other coexisting metal ions. In addition, this composite exhibited the dynamic nature of quenching in the presence of Cr3+ ion, which is confirmed by the Stern–Volmer plot, fluorescence temperature profiles, and decay time experiments. The GO-RhB nanocomposite-based fluorescent probe was successfully applied to the quantitative detection of Cr3+ ion in milk sample (linear range = 2 to 10 nM) with better performance than other existing methods. Besides, this GO-RhB composite showed better antibiofilm activity against Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus (MRSA) by using the Congo red agar and tube method.

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Funding

This work is supported by the SERB-EMR grant by the DST (Sanction No. SERB-EMR/2016/005692) and the Researchers Supporting Project number (RSP-2021/142), King Saud University, Riyadh, Saudi Arabia. The Center for Nanotechnology of Karunya Institute of Technology and Sciences supported the characterization techniques.

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Authors and Affiliations

  1. Fluorensic Materials Lab, Department of Applied Chemistry, Karunya Institute of Technology and Sciences (Declared As Deemed-To-Be University), Karunya Nagar, Coimbatore, 641114, India

    Krishnasamy Velmurugan, Nanjan Bhuvanesh & Raju Nandhakumar

  2. Department of Nanosciences and Technology, Karunya Institute of Technology and Sciences (Declared As Deemed-To-Be University), Karunya Nagar, Coimbatore, 641114, India

    Arul Felix Prakash

  3. Department of Applied Physics, Karunya Institute of Technology and Sciences (Declared As Deemed-To-Be University), Karunya Nagar, Coimbatore, 641114, India

    Velusamy Maheskumar & Bhojan Vidhya

  4. Department of Biotechnology, Karunya Institute of Technology and Sciences (Declared As Deemed-To-Be University), Karunya Nagar, Coimbatore, 641114, India

    Sevanan Murugan

  5. Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

    Raju Suresh Kumar & Abdulrahman I. Almansour

  6. Department of Chemistry and Biochemistry, The Ohio State University, 151 W. Woodruff Ave, Columbus, OH, 43210, USA

    Karthikeyan Perumal

Authors
  1. Krishnasamy Velmurugan
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  2. Nanjan Bhuvanesh
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  3. Arul Felix Prakash
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  4. Velusamy Maheskumar
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  5. Bhojan Vidhya
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  6. Sevanan Murugan
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  7. Raju Suresh Kumar
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  8. Abdulrahman I. Almansour
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  9. Karthikeyan Perumal
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  10. Raju Nandhakumar
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Correspondence to Bhojan Vidhya, Sevanan Murugan or Raju Nandhakumar.

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Velmurugan, K., Bhuvanesh, N., Prakash, A.F. et al. Graphene oxide–rhodamine nanocomposite for picomolar detection of chromium(III) by fluorimetry and its biofilm inhibition. Microchim Acta 188, 414 (2021). https://doi.org/10.1007/s00604-021-05057-9

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  • DOI: https://doi.org/10.1007/s00604-021-05057-9

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