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N, O-codoped hierarchical porous graphitic carbon for electrochemical immunosensing of Lactobacillus rhamnosus GG

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Abstract

An ultrasensitive label-free electrochemical immunosensor was fabricated for quantitative detection of Lactobacillus rhamnosus GG (LGG). The N/O co-doped three-dimensional hierarchical porous graphitic (THPG) carbon was synthesized by a one-step synthesis of polyaniline hydrogel, and followed by simple carbonization and chemical activation procedures. Because of the unique structure design, the obtained THPG carbon networks possess an ultra-large specific surface area of 4859 m2 g−1 along with a class of highly graphitic carbons. The results offer an enormous surface area and excellent electrical conductivity for label-free electrochemical immunosensing of probiotic L. rhamnosus strain. Under optimal conditions, the immunosensor showed a good linear relationship between peak current and concentration of LGG (R2 = 0.9976), with a detection limit of 2 CFU mL−1. Furthermore, this label-free immunosensor also shows good specificity, long-term stability, and reliability, and could be applied to detect probiotic LGG in dairy products and drinks with satisfactory results. The present protocol was shown to be quite promising for practical screening and functional evaluation of probiotic products containing LGG.

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A ultrasensitive label-free electrochemical immunosensor based on THPG carbon was fabricated for detection of Lactobacillus rhamnosus GG.

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Acknowledgements

Much thanks to The Testing Center of Yangzhou University due to all the characterization.

Funding

The funding of this work was provided by Natural Science Foundation of Jiangsu Province (No. BK20180922 and BK20190890), Natural science fund for colleges and universities in Jiangsu Province (No. 19KJB550002), State Key Laboratory of Analytical Chemistry for Life Science (No. SKLACLS2001), Nanjing University.

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  1. College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, 225127, People’s Republic of China

    Yajun Gao, Jiamin Wang, Yitian Du, Chunsen Wu, Huaxiang Li, Zhenquan Yang & Zhiyan Chen

  2. College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu, 225002, People’s Republic of China

    Yajun Gao & Zhanjun Yang

  3. Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou, Jiangsu, 225127, People’s Republic of China

    Yajun Gao & Zhenquan Yang

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  1. Yajun Gao
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Gao, Y., Wang, J., Du, Y. et al. N, O-codoped hierarchical porous graphitic carbon for electrochemical immunosensing of Lactobacillus rhamnosus GG. Microchim Acta 189, 5 (2022). https://doi.org/10.1007/s00604-021-05049-9

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