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Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening

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Abstract

A highly sensitive electrochemical biosensors has been developed for the detection of multiplex micro ribonucleic acids (miRNAs) by modifying an electrode with reduced graphene oxide/poly(2-aminobenzylamine)/gold nanoparticles and adopting porous, hollow silver-gold nanoparticles as tagged labeling with metal ions. In addition, an anti-deoxyribonucleic acid (DNA)-RNA hybrid [S9.6] antibody was used to detect different hybridized capture DNAs and miRNAs that can detect multiple miRNAs simultaneously. The developed electrochemical platform exhibits high selectivity, stability, and sensitivity with a wide linear range from 1 fM to 10 nM and a low detection limit of 0.98 fM, 3.58 fM, and 0.25 fM for miRNA-155, miRNA-21, and miRNA-16, respectively. In addition, the proposed electrochemical biosensor capable for the simultaneous detection of miRNA-155, miRNA-16, and miRNA-21, which are breast cancer biomarkers, in normal human serum, can be adopted and potentially used for breast cancer screening.

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Acknowledgements

This work was supported by the National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Grant No. P1851652. TP would like to express her gratitude to the National Nanotechnology Center (NANOTEC) and the National Science and Technology Development Agency (NSTDA) for postdoctoral fellowship.

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  1. National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand

    Dechnarong Pimalai, Thitirat Putnin, Wassa Waiwinya, Chuleekorn Chotsuwan, Noppadol Aroonyadet & Deanpen Japrung

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  1. Dechnarong Pimalai
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Pimalai, D., Putnin, T., Waiwinya, W. et al. Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening. Microchim Acta 188, 329 (2021). https://doi.org/10.1007/s00604-021-04995-8

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