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Functional magnetic nanoparticle–based affinity probe for MALDI mass spectrometric detection of ricin B

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Abstract

The use of lactosylated Fe3O4 magnetic nanoparticles (MNP@LAC) has been explored as affinity probes against ricin B based on galactose-ricin B binding interactions. Lactose was bound onto the surface of aminated MNPs through the Maillard reaction. The enrichment of ricin B took ~1 h by incubating MNP@LAC with samples under shaking at room temperature, followed by magnetic isolation. The resultant MNP@LAC-ricin B conjugates were characterized by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The limit of detection toward ricin B was ~3 nM by using the developed method. It was possible to detect the peptides derived from the tryptic digest of trace ricin B (~0.39 nM) enriched by the MNP@LAC probes followed by tryptic digestion and MALDI-MS analysis. The feasibility of using the developed method for detection of ricin B from complex white corn starch samples spiked with trace ricin B was demonstrated.

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Acknowledgements

We thank the Ministry of Science and Technology of Taiwan (MOST 108-2113-M-009-018-MY3) for the financial support of this research. KS thank the Ministry of Science and Technology of Taiwan for providing him the postdoctoral fellowship (MOST 109-2811-M-009-519).

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Authors and Affiliations

  1. Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 300, Taiwan

    Karthikeyan Kandasamy, Karuppuchamy Selvaprakash & Yu-Chie Chen

  2. Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan

    Karthikeyan Kandasamy, Karuppuchamy Selvaprakash & Yu-Chie Chen

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  1. Karthikeyan Kandasamy
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Correspondence to Yu-Chie Chen.

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Kandasamy, K., Selvaprakash, K. & Chen, YC. Functional magnetic nanoparticle–based affinity probe for MALDI mass spectrometric detection of ricin B. Microchim Acta 188, 339 (2021). https://doi.org/10.1007/s00604-021-04991-y

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