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A dual signal-amplified electrochemiluminescence immunosensor based on core-shell CeO2-Au@Pt nanosphere for procalcitonin detection

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Abstract

A dual signal-amplified sandwich electrochemiluminescence (ECL) immunosensor was fabricated for trace detection of procalcitonin (PCT). CeO2-Au@Pt composed of sea urchin-like Au@Pt nanoparticles coated on CeO2 hollow nanospheres was immobilized on electrode surface to electrochemically catalyze H2O2 to produce a large number of superoxide anion (O2•−). The immunosensor was prepared by linking the capture antibody on immobilized CeO2-Au@Pt with heptapeptide (HWRGWVC), which could maintain the activity of the antibody. The prepared Au star@BSA was used to bind abundant luminol for labeling the secondary antibody (Ab2). Upon the sandwich-typed immunoreactions, the O2•− could react with the introduced luminol on the immunosensor surface to produce strong ECL intensity. With an outstanding linear detection range and a low detection limit of 17 fg/mL, the ECL immunosensor permitted ultrasensitive detection of PCT at a low H2O2 concentration and demonstrated its high application potential in the clinical assay.

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Funding

This research was financially supported by the National Natural Science Foundation of China (Nos. 21777056, 21675063), the Innovation Team Project of Colleges and Universities in Jinan (No. 2019GXRC027), the Special Foundation for Taishan Scholar Professorship of Shandong Province, and the Jinan Scientific Research Leader Workshop Project (2018GXRC021, 2018GXRC024).

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Authors and Affiliations

  1. Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China

    Xinrong Shao, Xianzhen Song, Xin Liu, Lei Liu, Dawei Fan, Qin Wei & Huangxian Ju

  2. Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China

    Xinrong Shao, Xianzhen Song, Xin Liu, Lei Liu, Dawei Fan, Qin Wei & Huangxian Ju

  3. School of Water Conservancy and Environment, University of Jinan, Jinan, 250022, People’s Republic of China

    Liangguo Yan

  4. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, People’s Republic of China

    Huangxian Ju

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  1. Xinrong Shao
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  2. Xianzhen Song
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Correspondence to Dawei Fan.

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Shao, X., Song, X., Liu, X. et al. A dual signal-amplified electrochemiluminescence immunosensor based on core-shell CeO2-Au@Pt nanosphere for procalcitonin detection. Microchim Acta 188, 344 (2021). https://doi.org/10.1007/s00604-021-04988-7

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