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Design of Gd3+-immobilized two-dimensional magnetic magadiite nanosheets for highly selective enrichment of phosphopeptides

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Abstract

Exfoliated magadiite nanosheets embedded with Fe3O4 were constructed. Advantage was taken of the strong coordination between the silanol groups in magadiite nanosheets and the Gd3+ ion to prepare the final adsorbent, Gd3+-immobilized magnetic magadiite nanosheets. The adsorbent with two-dimensional (2D) morphology offered high surface area and abundant Gd3+ contents for phosphopeptides enrichment, on which Fe3O4 with positive electricity incorporated the magnetic properties. Combining with matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF-MS), the method showed low detection limit (0.05 fmol). The feasibility of using the 2D nanocomposite for phosphopeptides enrichment was demonstrated using mixtures of β-casein and bovine serum albumin (1:5000). The standard deviation of captured phosphopeptides in three repeated experiments were in the range 0.15–0.42 (< 0.5% RSD). Further evaluation revealed that the nanocomposite was capable of enriching phosphopeptides from non-fat milk, human saliva, and serum.

Graphical abstract

A novel Gd3+-immobilized two-dimensional magnetic magadiite nanosheets-based enrichment platform was designed. The developed material was employed as the adsorbent for the selective enrichment of phosphopeptides by coupling with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The material was successfully applied to enrich phosphopetides from standard peptide mixtures, nonfat milk, human saliva, and serum.

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Acknowledgement

This work was financially supported by the National Natural Science Foundation of China (22004073, 21961024, and 21961025), Natural Science Foundation of Inner Mongolia (2020BS02010, 2020BS02015, and 2018JQ05), Talent research support funds from government-sponsored institution of Inner Mongolia (RCQD19002), Inner Mongolia Autonomous Region Funding Project for Science & Technology Achievement Transformation (CGZH2018156), and IMUN Doctoral Research Startup Fund Project (BS516 and BS513).

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Authors and Affiliations

  1. College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), Inner Mongolia University for Nationalities, Tongliao, 028000, People’s Republic of China

    Dandan Jiang, Siqi Lv, Xue Han, Limei Duan & Jinghai Liu

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  1. Dandan Jiang
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  2. Siqi Lv
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  3. Xue Han
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  4. Limei Duan
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  5. Jinghai Liu
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Correspondence to Dandan Jiang or Jinghai Liu.

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Jiang, D., Lv, S., Han, X. et al. Design of Gd3+-immobilized two-dimensional magnetic magadiite nanosheets for highly selective enrichment of phosphopeptides. Microchim Acta 188, 327 (2021). https://doi.org/10.1007/s00604-021-04972-1

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