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Combination of ultrathin micro-patterned MXene and PEDOT: Poly(styrenesulfonate) enables organic electrochemical transistor for amperometric determination of survivin protein in children osteosarcoma

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Abstract

An ultrathin micro-patterned MXene/PEDOT:PSS-based organic electrochemical transistor biosensor was constructed, which can significantly amplify the amperometric signal and transistor’s performance. A novel interdigitated OECTs biosensor has been developed for reliable determination of survivin for the following considerations: (1) The synergistic effect of intercalated MXene and ionic PEDOT:PSS enhanced the mobility and volumetric capacitance of OECTs biosensor. (2) Compared with the best previous literatures, our assay demonstrated enhanced detection limit of survivin down to 10 pg mL−1, as well as satisfactory selectivity, reproducibility, and reliability. (3) Comparison of OECTs against commercial ELISA kit yielded favorable linearity (Y = 1.0015*X + 0.0039) and correlation coefficient (R2 = 0.9717). Those advantages are expected to pave the way to design of an OECTs biosensor with robustness, non-invasiveness, and miniaturization for the point-of-care applications.

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Funding

The research leading to these results received funding from Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition (17DZ2272000).

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  1. Ping Xu and Chunwen Lu contributed equally to this work.

Authors and Affiliations

  1. Department of Orthopedics, Children’s Hospital of Fudan University, National Children’s Medical Center, Shanghai, China

    Ping Xu, Dahui Wang & Dong Fu

  2. Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai, China

    Chunwen Lu

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  1. Ping Xu
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Contributions

Ping Xu: Conceptualization, methodology, investigation, writing-original draft, and formal analysis. Chunwen Lu: Investigation, writing-original draft, and software. Dahui Wanga: Writing-original draft. Dong Fu: Methodology, supervision, and writing-review and editing.

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Correspondence to Dahui Wang or Dong Fu.

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Xu, P., Lu, C., Wang, D. et al. Combination of ultrathin micro-patterned MXene and PEDOT: Poly(styrenesulfonate) enables organic electrochemical transistor for amperometric determination of survivin protein in children osteosarcoma. Microchim Acta 188, 301 (2021). https://doi.org/10.1007/s00604-021-04947-2

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