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Amplified electrochemical antibiotic aptasensing based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework

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Abstract

A facile and versatile competitive electrochemical aptasensor for tobramycin (TOB) detection is described using electrochemical-deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework (AuNPs/P-MOF) as signal-amplification platform and a DNA probe labeled with methylene blue (MB) at the 3′-end (MB-Probe) as a signal producer. First, F-Probe (short complementary DNA strands of both the aptamer and the MB-Probe label with a sulfhydryl group at the 5′-end) was immobilized on the AuNPs/P-MOF modified electrode as detection probes, which competed with TOB in binding to the aptamer. TOB-aptamer binding resulted in F-Probe remaining unhybridized on the electrode surface, so that a significant current response was generated by hybridizing with MB-Probe instead. The developed strategy showed favorable repeatability, with a relative standard deviation (RSD) of 4.3% computed over five independent assays, and high stability, with only 6.8% degradation after 15 days of storage. Under optimal conditions, the proposed aptamer strategy exhibited a linear detection range from 100 pM to 500 nM with a limit of detection (LOD) of 56 pM (S/N = 3). The electrochemical aptasensor demonstrated remarkable selectivity, and its feasibility for accurate and quantitative detection of TOB in milk samples was confirmed (RSD < 4.5%). Due to its simple design, easy operation, and high sensitivity and selectivity, the proposed method could expect to detect other antibiotics by replacing the aptamers. In summary, this study provides a simple and effective new strategy for electrochemical aptasening based on MOF-based sensing interface.

Graphical abstract

Scheme illustration of label-free competitive electrochemical aptamer-based detection of tobramycin based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework as signal-amplification platform.

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Funding

This work was supported by the National Natural Science Foundation of China (31901783); Postdoctoral Science Foundation of China (2019M652835); National Key R&D Program of China (2017YFC1600403), Guangdong Provincial Key Laboratory (2020B121201009) and GDAS’ Project of Science and Technology Development (2019GDASYL0103006, 2020GDASYL-0301002).

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Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, People’s Republic of China

    Youxiong Zhang, Bing Li, Xianhu Wei, Qihui Gu, Moutong Chen, Jumei Zhang, Shuping Mo, Liang Xue & Qingping Wu

  2. College of Food Science, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Bing Li & Juan Wang

  3. Department of Food Science & Technology, Institute of Food Safety and Nutrition, Jinan University, Guangzhou, 510632, People’s Republic of China

    Yu Ding

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  1. Youxiong Zhang
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Zhang, Y., Li, B., Wei, X. et al. Amplified electrochemical antibiotic aptasensing based on electrochemically deposited AuNPs coordinated with PEI-functionalized Fe-based metal-organic framework. Microchim Acta 188, 286 (2021). https://doi.org/10.1007/s00604-021-04912-z

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