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Nanozyme based on graphene oxide modified with Fe3O4, CuO, and cucurbit[6]uril for colorimetric determination of homocysteine

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Abstract

A nanozyme based on graphene oxide modified with Fe3O4 NPs, CuO NPs, and cucurbit[6]uril has been successfully fabricated by a simple sonochemical technique. By employing CB[6] as a specific binding pocket and Fe3O4@CuO-GO as a peroxidase mimic, this novel nanozyme (BN I) is equipped with molecular recognition ability and enhanced peroxidase-like activity. On the basis of the inhibition effect of homocysteine (Hcy) towards the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) catalyzed by BN I, a simple colorimetric method is established for the sensitive and selective determination of Hcy. This proposed method displays a good linear response in the range 5–200 μM with a detection limit of 1.8 μM. In the practical assay of human plasma samples, the relative standard deviations (RSD) are lower than 11% and the recoveries are between 98.0 and 104.9%. In the assay of human urine samples, the RSD are below 9.0% and the recoveries range from 94.0 to 103.5%. The colorimetric method presented offers a convenient and accurate way for the determination of biomarkers in point-of-care testing (POCT).

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Funding

This work was financially supported by the Independent Innovation Fund Project of Agricultural Science and Technology of Jiangsu Province in 2017 (No. CX (17) 1003), the Natural Science Foundation of China Projects (No. 81950410634, No. QNJ20200010003) for a Foreign Youth Project fund, and Innovation and Entrepreneurship Training Program for Undergraduate (No. 202010316209).

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Authors and Affiliations

  1. Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 211100, China

    Zhaorui Song, Chenrui Jiang, Fangqi Wang, Lili Yu, Sijing Ye, Pierre Dramou & Hua He

  2. Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, 211100, China

    Hua He

  3. Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing, 211198, China

    Hua He

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  1. Zhaorui Song
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Correspondence to Pierre Dramou or Hua He.

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Song, Z., Jiang, C., Wang, F. et al. Nanozyme based on graphene oxide modified with Fe3O4, CuO, and cucurbit[6]uril for colorimetric determination of homocysteine. Microchim Acta 188, 207 (2021). https://doi.org/10.1007/s00604-021-04868-0

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