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Gold-silver core-shell nanoparticle–based impedimetric immunosensor for detection of iron homeostasis biomarker hepcidin

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Abstract

An impedimetric immunosensor based on gold-silver core-shell nanoparticles for hepcidin detection is reported. The core-shell nanoparticles were prepared by seed-mediated method and characterized by dynamic light scattering, UV-Vis, XRD, field emission-scanning electron micrograph imaging, energy dispersive spectroscopy, and atomic force microscopy. The immunosensor was fabricated with core-shell nanoparticles and cysteamine employing covalent chemistry (amide bond formation) strategy for ensuring proper orientation of anti-hepcidin antibody on to the amine-functionalized nanomaterial decorated electrodes. The hepcidin detection principle was based on the variation of charge transfer resistance (ΔRct) relative to the Fe(CN)64−/3− electrochemical probe in the presence of the biomarker. The frequency range was 10−1 to 105 Hz at the scan rate of 10 mV s−1and a potential of 0.1 V. Based on the antigen-antibody interaction in 40 min at pH 7.0, a linear relationship between ΔRct and hepcidin concentration was obtained in the range 0.01 to 100 ng/mL with a detection limit of 0.857 pg/mL. Furthermore, the designed immunosensor had acceptable reproducibility, stability, selectivity, and reusability. It was successfully applied to the detection of hepcidin in spiked human serum samples and acceptable recovery (90–95.9%) was obtained.

Gold-silver core-shell nanoparticle–based impedimetric immunosensor for detection of iron homeostasis biomarker hepcidin. The study focuses on the detection of iron regulatory protein hepcidin using gold-silver core-shell nanoparticles. This immunosensor was fabricated with core-shell nanoparticles and cysteamine employing covalent chemistry (amide bond formation) strategy. The sensor was sensitive in the range from 0.01 to 100 ng/mL, with a detection limit of 0.857 pg/mL.

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Acknowledgments

The authors acknowledge the Central Instrument Laboratory, Panjab University, for instrumentation facilities. I would also like to thank and acknowledge UGC-NET for providing the award of fellowship [19/06/2016(i)-EU-V].

Funding

The research was financially supported by DST-SERB [EEQ/2017/000239], DST-Purse II, and Special Assistance Programme (UGC-SAP) [F.4-7/2015/DRS-III (SAP-II)].

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Authors and Affiliations

  1. Department of Biochemistry, Panjab University, Sector-25, Chandigarh, 160014, India

    Shilpa Rana, Anu Bharti, Archana Bhatnagar & Nirmal Prabhakar

  2. CSIR-Central Scientific Instruments Organization, Sector-30-C, Chandigarh, 160030, India

    Suman Singh

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  1. Shilpa Rana
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Correspondence to Nirmal Prabhakar.

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Rana, S., Bharti, A., Singh, S. et al. Gold-silver core-shell nanoparticle–based impedimetric immunosensor for detection of iron homeostasis biomarker hepcidin. Microchim Acta 187, 626 (2020). https://doi.org/10.1007/s00604-020-04599-8

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