Based on the hydrogel-AuNP supramolecular sphere (H-Au), a label-free and real-time surface plasmon resonance imaging biosensor has been developed for highly sensitive and specific determination of prostate cancer cell-derived exosomes. After integrating the signal amplification effect of the mass cumulative hydrogel and the LSPR effect of AuNPs with high specific aptamer, the SPRi biosensor for exosome detection exhibited a wide linear range from 1.00 × 105 to 1.00 × 107 particles/mL with a limit of detection of 1.00 × 105 particles/mL. Most importantly, with a strong correlation between the SPRi signal and the t-PSA value measured by the clinical chemiluminescence immunoassay, this biosensor displayed excellent practicability for human serum analysis, which exhibits great potential applications in disease diagnosis and bioanalysis.
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Shoag J, Sedrakyan A, Halpern J, Hsu WC, Hu J (2017) Mp14-15 increase in the incidence of advanced prostate cancer in the United States. Int J Urol 197:e167
Lin L, Yan L, Liu Y (2019) Incidence and death in 29 cancer groups in 2017 and trend analysis from 1990 to 2017 from the Global Burden of Disease Study. J Hematol Oncol 12(1):1–21
Ost P, Bossi A, Decaestecker K, De Meerleer G, Giannarini G, Karnes RJ (2015) Metastasis-directed therapy of regional and distant recurrences after curative treatment of prostate cancer: a systematic review of the literature. Eur Urol 67:852–863
Cai G, Yu Z, Ren R (2018) Exciton-plasmon interaction between AuNPs/Graphene nanohybrids and CdS quantum dots/TiO2 for photoelectrochemical aptasensing of prostate-specific antigen. Acs Sens 3:632–639
Feng S, Lian Z, Lei J (2018) An enzyme-free immunosorbent assay of prostate specific antigen amplified by releasing pH indicator molecules entrapped in mesoporous silica nanoparticles. Anal Chem 90:7086
Cheng N, Du D, Wang X, Liu D, Xu W, Luo Y, Lin Y (2019) Recent advances in biosensors for detecting cancer-derived exosomes. Trends Biotechnol 37(11):1236–1254
Xu H, Ye BC (2020) Advances in biosensing technologies for analysis of cancer-derived exosomes. TrAC Trends Anal Chem 123:115773
Shao B, Xiao Z (2020) Recent achievements in exosomal biomarkers detection by nanomaterials-based optical biosensors-a review. Anal Chim Acta 1114(1):74–84
Sokolova V, Ludwig AK, Hornung S (2011) Characterization of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy. Colloids Surf B Biointerfaces 87(1):146–150
Zhang P, He M, Zeng Y (2016) Ultrasensitive microfluidic analysis of circulating exosomes using a nanostructured graphene oxide/polydopamine coating. Lab Chip 16(16):3033–3042
Vand P, Hoekstra AG, Sturk A (2010) Optical and non-optical methods for detection and characterization of microparticles and exosomes. J Thromb Haemost 8(12):2596–2607
Zhu L, Wang K, Cui J, Liu H, Bu X, Ma H (2014) Label-free quantitative detection of tumor-derived exosomes through surface plasmon resonance imaging. Anal Chem 86(17):8857–8864
Im H, Shao H, Park YI (2014) Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor. Nat Biotechnol 32(5):490–495
Rupert D, Lässer C, Eldh M (2014) Determination of exosome concentration in solution using surface plasmon resonance spectroscopy. Anal Chem 86(12):5929–5936
Christopher L, Hu Z, Hood L (2014) SPR imaging for high throughput, label-free interaction analysis. Comb Chem High Throughput Screen 12(8):741–751
Campbell C, Kim G (2007) SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics. Biomaterials 28(15):2380–2392
Schneider A, Simons M (2013) Exosomes: vesicular carriers for intercellular communication in neurodegenerative disorders. Cell Tissue Res 352(1):33–47
Matsishin M, Rachkov A, Lopatynskyi A (2017) Selective amplification of SPR biosensor signal for recognition of rpoB gene fragments by use of gold nanoparticles modified by thiolated DNA. Nanoscale Res Lett 12(1):252
Vaisocherová H, Šípová H, Víšová I (2015) Rapid and sensitive detection of multiple microRNAs in cell lysate by low-fouling surface plasmon resonance biosensor. Biosens Bioelectron 70:226–231
Liu Y, Cheng Q (2012) Detection of membrane-binding proteins by surface plasmon resonance with an all-aqueous amplification scheme. Anal Chem 84(7):3179–3186
Karczmarczyk A, Reinerrozman C, Hageneder S (2016) Fast and sensitive detection of ochratoxin A in red wine by nanoparticle-enhanced SPR. Anal Chim Acta 937:143–150
Fang S, Lee H, Wark A (2006) Attomole microarray detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions. J Am Chem Soc 128(43):14044–14046
Nie W, Wang Q, Zou L (2018) Low-fouling surface plasmon resonance sensor for highly sensitive detection of microRNA in a complex matrix based on the DNA tetrahedron. Anal Chem 90(21):12584–12591
Wu Q, Li N, Wang Y (2020) Ultrasensitive and selective determination of carcinoembryonic antigen using multifunctional ultrathin amino-functionalized Ti3C2-MXene nanosheets. Anal Chem 92(4):363
Guo B, Wen B, Cheng W (2018) An enzyme-free and label-free surface plasmon resonance biosensor for ultrasensitive detection of fusion gene based on DNA self-assembly hydrogel with streptavidin encapsulation. Biosens Bioelectron 112:120–126
Yan C, An P (2019) Concatenated catalytic hairpin assembly/hyperbranched hybridization chain reaction based enzyme-free signal amplification for the sensitive photoelectrochemical detection of human telomerase RNA. Anal Chem 91:2447
Ding X, Cheng W, Li Y (2017) An enzyme-free surface plasmon resonance biosensing strategy for detection of DNA and small molecule based on nonlinear hybridization chain reaction. Biosens Bioelectron 87:345–351
Andersson O, Larsson A, Ekblad T (2009) Gradient hydrogel matrix for microarray and biosensor applications: an imaging SPR study. Biomacromolecules 10(1):142–148
Toma M, Jonas U, Dostálek J (2013) Active control of SPR by thermoresponsive hydrogels for biosensor applications. J Phys Chem C 117(22):11705–11712
Beines P, Klosterkamp I, Menges B (2007) Responsive thin hydrogel layers from photo-cross-linkable poly (N - isopropylacrylamide) terpolymers. Langmuir 23(4):2231–2238
You Y, Lim S, Gunasekaran S (2020) Streptavidin-coated Au nanoparticles coupled with biotinylated antibody-based bifunctional linkers as plasmon-enhanced immunobiosensors. ACS Appl Nano Mater 3(2):1900–1909
Mathias R, Lim J, Ji H (2009) Isolation of extracellular membranous vesicles for proteomic analysis. Methods Mol Biol 528:227–242
Liu J, Lu Y (2006) Preparation of aptamer-linked gold nanoparticle purple aggregates for colorimetric sensing of analytes. Nat Protoc 1(1):246–252
Dong H, Chen H, Jiang J (2018) Highly sensitive electrochemical detection of tumor exosomes based on aptamer recognition-induced multi-DNA release and cyclic enzymatic amplification. Anal Chem 90(7):4507–4513
Yu T, Dai P, Xu J (2016) Highly sensitive colorimetric cancer cell detection based on dual signal amplification. ACS Appl Mater Interfaces 8(7):4434–4441
Liu T, Mendes DE, Berkman CE (2014) Functional prostate-specific membrane antigen is enriched in exosomes from prostate cancer cells. Int J Oncol 44(3):918–922
Boyacioglu O, Stuart CH, Kulik G (2013) Dimeric DNA aptamer complexes for high-capacity-targeted drug delivery using pH-sensitive covalent linkages. Mol Ther Nucleic Acids 2(7):e107
Huang Z, Lin Q, Ye X (2020) Terminal deoxynucleotidyl transferase based signal amplification for enzyme-linked aptamer-sorbent assay of colorectal cancer exosomes. Talanta 2020(218):121089
Lee J, Kim J, Kwon M (2015) In situ single step detection of exosome microRNA using molecular beacon. Biomaterials 54:116–125
Sina A, Vaidyanathan R, Wuethrich A (2019) Label-free detection of exosomes using a surface plasmon resonance biosensor. Anal Bioanal Chem 411(7):1311–1318
Cheng B, Fei T, Cui N (2018) Highly sensitive detection of exosomes by SERS using gold nanostar@Raman reporter@nanoshell structures modified with a bivalent cholesterol-labeled DNA anchor. Analyst 143(20):4915–4922
Fu JL, Fang Q, Zhang T (2006) Laser-induced fluorescence detection system for microfluidic chips based on an orthogonal optical arrangement. Anal Chem 78(11):3827–3834
Vaidyanathan R, Naghibosadat M, Rauf S (2014) Detecting exosomes specifically: a multiplexed device based on alternating current electrohydrodynamic induced nanoshearing. Anal Chem 86(22):11125–11132
Wang H, Chen H, Huang Z (2018) DNase I enzyme-aided fluorescence signal amplification based on graphene oxide-DNA aptamer interactions for colorectal cancer exosome detection. Talanta 184:219–226
Zhou Q, Rahimian A, Son K (2016) Development of an aptasensor for electrochemical detection of exosomes. Methods 97:88–93
Liu N, Ma Z (2014) Au-ionic liquid functionalized reduced graphene oxide immunosensing platform for simultaneous electrochemical detection of multiple analytes. Biosens Bioelectron 51(1):184–190
Zhang D, Yan Y, Cheng W (2015) Streptavidin-enhanced surface plasmon resonance biosensor for highly sensitive and specific detection of microRNA. Microchim Acta 180(5–6):397–403
This work was supported by the National Natural Science Foundation of China (81873980, 81873972), the financial support from the National Science and Technology Major Project of the Ministry of Science and Technology of China (2018ZX10732202), and the Chongqing Medical University Graduate Talent Training Program (BJRC201908).
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Chen, W., Li, J., Wei, X. et al. Surface plasmon resonance biosensor using hydrogel-AuNP supramolecular spheres for determination of prostate cancer-derived exosomes. Microchim Acta 187, 590 (2020). https://doi.org/10.1007/s00604-020-04573-4
- Surface plasmon resonance imaging (SPRi)
- Hydrogel-gold nanoparticles (hydrogel-AuNPs)
- Tumor diagnosis
- Prostate-specific antigen (PSA)