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Colorimetric and fluorescent dual-identification of glutathione based on its inhibition on the 3D ball-flower shaped Cu-hemin-MOF’s peroxidase-like activity

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Abstract

Hemin as the organic linker ligand and Cu (II) as the metal center were applied to prepare a copper-metal-organic framework (Cu-hemin-MOF) via one-step hydrothermal method. Characterization using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD) demonstrate that the acquired Cu-hemin-MOF possesses the appearance of 3D ball-flower shape with the existence of C, N, O, Fe, and Cu on the surface. Further study found that this 3D ball-flower shaped Cu-hemin-MOF exhibits peroxidase-like activity, which can catalyze the peroxidase substrate of o-phenylenediamine (OPD) to generate 2,3-diaminophenazine (DAP) in the presence of H2O2. DAP has a yellow color and also emits a strong fluorescence when excited by ultraviolet light. Interestingly, Cu-hemin-MOF’s peroxidase-like activity can be strongly inhibited by glutathione (GSH). In view of this, a dual readout (fluorescence and colorimetry) was proposed to detect GSH for the first time. Under optimal conditions, the proposed method exhibits good linear relationship between the signal response (fluorescence and colorimetry) and the concentration of GSH, and low limits of detection (LOD) of 2.3 and 26.6 nM, respectively.

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Funding

This work was supported by the National Natural Science Foundation of China [grant numbers 81772290 and 81271930]; Graduate Scientific Research and Innovation Foundation of Chongqing, China [grant number CYB19041],;Fundamental Research Funds for the Central Universities [grant numbers 2019CDYGZD007 and 2020CDCGSW052]; Brew Microorganisms Technology and Application of Key Laboratory Project in Sichuan Province [grant number, NJ2018-01]; Chongqing science and technology commission [grant number CSTC2018jcyjAX0062]; Strong-Flavor Baijiu Solid-State Fermentation Key Laboratory of China light industry [grant number 2019JJ002]; Chongqing Graduate Tutor Team Construction Project, Analytical, and Testing Center of Chongqing University for SEM, FT-IR, XRD, and XPS; and the sharing fund of Chongqing University’s large equipment for financial support.

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Author notes

  1. Xiaolong Chen and Xianfeng Wang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory for Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, People’s Republic of China

    Xiaolong Chen, Xianfeng Wang, Gaihua Cao, Yawen Wu, Zhong Ji, Caihong Shen, Danqun Huo & Changjun Hou

  2. Liquor Making Biology Technology and Application of Key Laboratory of Sichuan Province, College of Bioengineering, Sichuan University of Science and Engineering, Zigong, 643000, People’s Republic of China

    Huibo Luo & Danqun Huo

  3. National Engineering Research Center of Solid-State Brewing, Luzhou Laojiao Group Co. Ltd., Luzhou, 646000, People’s Republic of China

    Caihong Shen

  4. Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, 400044, People’s Republic of China

    Changjun Hou

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  1. Xiaolong Chen
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Chen, X., Wang, X., Cao, G. et al. Colorimetric and fluorescent dual-identification of glutathione based on its inhibition on the 3D ball-flower shaped Cu-hemin-MOF’s peroxidase-like activity. Microchim Acta 187, 601 (2020). https://doi.org/10.1007/s00604-020-04565-4

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