An electrochemical method has been developed to determine iodide in urine using an electrode modified with silver oxide microparticles–poly acrylic acid/poly vinyl alcohol (Ag2OMPs-PAA/PVA). Silver oxide particles were formed by electrochemical oxidation via cyclic voltammetry. The modified electrode exhibited an excellent response to iodide detection by cathodic stripping voltammetry. The fabrication and operation conditions were optimized in terms of PVA concentration, K2HPO4 concentration, amount of AgMPs-PAA/PVA, number of cycles for oxide formation, electrolyte, applied potential (vs. Ag/AgCl), and time. Under the optimum conditions, iodide determination produced a linear range from 1 to 40 μM. The limit of detection was 0.3 μM. Precision was found to be within 7.4% RSD. The developed method was applied to the determination of iodide in urine samples of pregnant women with satisfying recoveries (86 ± 1 to 108 ± 1%).
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The authors would like to thank Mr.Thomas Duncan Coyne for his help in preparing the manuscript.
The authors received financial support from the Thailand Research Fund, Prince of Songkla University and Faculty of Science, Prince of Songkla University (Grant No. BRG6080009); the Center of Excellence for Innovation in Chemistry (PERCH-CIC); and the Ministry of Higher Education, Science, Research and Innovation; the Trace Analysis and Biosensor Research Center (TAB-RC), Prince of Songkla University; and the Department of Applied Science, Department of Chemistry Faculty of Science and Graduate School, Prince of Songkla University, Hat Yai, Songkhla.
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Khunseeraksa, V., Kongkaew, S., Thavarungkul, P. et al. Electrochemical sensor for the quantification of iodide in urine of pregnant women. Microchim Acta 187, 591 (2020). https://doi.org/10.1007/s00604-020-04488-0
- Electrochemical iodide sensor
- Urine analysis
- Pregnant women
- Silver oxide microparticles
- Cathodic stripping voltammetry