Photoelectrochemical immunoassay platform based on MoS2 nanosheets integrated with gold nanostars for neuron-specific enolase assay

Abstract

MoS2 nanosheets were prepared by exfoliating MoS2 bulk crystals with ultrasonication in N-methylpyrrolidone and were integrated with gold nanostars (AuNS) to fabricate an AuNS/MoS2 nanocomposite. All nanomaterials were characterized by transmission electron microscope, scanning electron microscope, ultraviolet-visible spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. AuNS/MoS2 nanocomposites were coated onto a glassy carbon electrode (GCE) surface to construct a nanointerface for immobilizing neuron-specific enolase antibody (anti-NSE) thus forming a photoelectrochemical immunoassay system. AuNS can significantly promote the photoelectric conversion of MoS2 nanosheets improving the performance for a photoelectrochemical assay. Being illuminated with white light LED and controlling the potential at 0.05 V (vs. SCE), the photocurrent generated from anti-NSE(BSA)/AuNS/MoS2/GCE using 0.15 mol L−1 ascorbic acid as electron donor can be recorded with amperometry and used as an output signal for NSE quantitative assay. Under optimized experimental conditions, the photocurrent variation for the affinity-binding NSE is proportional to the logarithm of NSE concentration in the range 5.0 pg mL-1   to 1.5 ng mL−1 with a detection limit of 3.5 pg mL−1 (S/N = 3). The practicability of the PEC immunoassay system was evaluated by determining NSE in clinical serum samples. The recoveries ranged from 93.0 to 103% for the determination of NSE in serum samples with a standard addition method. The PEC immunoassay system possesses good accuracy for determining NSE in real samples.

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Funding

The authors gratefully acknowledge the financial supports from the National Natural Science Foundation of China (No. 21675175, 21874157 and 21275166), Major Projects of Technical Innovation of Hubei Province (No. 2017ACA172), the Natural Science Foundation of Hubei Province (No. 2018CFB617 and 2015CFA092), and Jiangmen Program for Innovative Research Team (No. 2018630100180019806).

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Correspondence to Haiyan Li or Chunya Li.

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Liu, R., Wang, Y., Wong, W. et al. Photoelectrochemical immunoassay platform based on MoS2 nanosheets integrated with gold nanostars for neuron-specific enolase assay. Microchim Acta 187, 480 (2020). https://doi.org/10.1007/s00604-020-04411-7

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Keywords

  • Photoelectrochemical immunoassay
  • Neuron-specific enolase
  • MoS2 nanosheet
  • Gold nanostar