A sandwich-type fluorescent biosensor for the determination of tumor-related exosome was designed. It is based on magnetic nanoparticle (MNP) capture and horseradish peroxidase (HRP) catalysis. MNPs were used as the substrate to capture exosomes by modifying the CD63 antibody on MNPs surface. After that, the biotinylated epithelial cell adhesion molecule (EpCAM) antibody was used to capture the tumor-related exosomes, which specifically express EpCAM. A novel method for the fluorescence measurement of tumor-associated exosome was achieved, with a detection limit as low as 200 (± 9) particles mL−1. The analytical range of this method is from 576 (± 15) particles mL−1 to 5.76 × 107 (± 5.1 × 105) particles mL−1. For the fluorescence measurement, the excitation wavelength was set to 320 nm. Fluorescent spectra were collected at emission wavelength in the range 370 to 550 nm; the data shown in the calibration plot were studied by using the fluorescence intensity at 406 nm. This sensor was also able to successfully detect the exosomes from the plasma of patients with hepatocellular carcinoma (HCC) and healthy humans.
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These works were supported by the National Natural Science Foundation of China (81872509), the Hubei Provincial Technology Innovation Project (2017ACA176), the Free Exploration Project of Hubei University of Medicine (FDFR201804), the Open Project of Hubei Key Laboratory of Wudang Local Chinese Medicine Research (Hubei University of Medicine) (Grant No. WDCM2018004), the Research Project of Traditional Chinese Medicine of the Foundation of Health Commission of Hubei Province (ZY2019M034), the Scientific and Technology Project of Shiyan City of Hubei Province (Nos. 19Y87, 19Y88, and 19Y93), the Key Discipline Project of Hubei University of Medicine and Hubei Province health and family planning scientific research project (WJ2019M054), and the Innovative Research Program for Graduates of Hubei University of Medicine (YC2019021).
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Chen, H., Luo, D., Shang, B. et al. Immunoassay-type biosensor based on magnetic nanoparticle capture and the fluorescence signal formed by horseradish peroxidase catalysis for tumor-related exosome determination. Microchim Acta 187, 282 (2020). https://doi.org/10.1007/s00604-020-04275-x
- Fluorescent biosensor
- Cancer diagnosis
- Sandwich structure