Skip to main content
SpringerLink
Log in

Fluorometric determination of lead(II) by using aptamer-functionalized upconversion nanoparticles and magnetite-modified gold nanoparticles

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

A fluorescent nanoprobe for Pb(II) has been developed by employing aptamer-functionalized upconversion nanoparticles (UCNPs) and magnetic Fe3O4-modified (MNPs) gold nanoparticles (GNPs). First, aptamer-functionalized UCNPs and aptamer-functionalized magnetic GNPs were synthesized to obtained the fluorescent nanoprobe. The particles were combined by adding a complementary ssDNA. In the absence of Pb(II), the UCNPs, MNPs and GNPs are linked via complementary base pairing. This led to a decrease in the green upconversion fluorescence peaking at 547 nm (under 980 nm excitation). In the presence of Pb(II), the dsDNA between UCNPs and MNPs-GNPs is cleaved, and fluorescence recovers. This effect allows Pb(II) to be quantified, with a wide working range of 25–1400 nM and a lower detection limit of 5.7 nM. The nanoprobe gave satisfactory results when analyzing Pb(II) in tea and waste water.

Schematic representation of fluorescent nanoprobe based on fluorescence resonance energy transfer (FRET) between upconversion nanoparticles (UCNPs) and gold nanoparticles (GNPs)-Fe3O4 magnetic nanoparticles (MNPs) for detection of Pb2+.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig.3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Wen B, Xue J, Zhou X, Wu Q, Nie J, Xu J, Du B (2018) Highly selective and sensitive detection of Pb2+ in aqueous solution using tetra(4-pyridyl)porphyrin-functionalized Thermosensitive ionic microgels. ACS Appl Mater Interfaces 10:25706–25716

    Article  CAS  Google Scholar 

  2. Niu X, Zhong Y, Chen R, Wang F, Liu Y, Luo D (2018) A “turn-on” fluorescence sensor for Pb2+ detection based on graphene quantum dots and gold nanoparticles. Sensors Actuators B Chem 255:1577–1581

    Article  CAS  Google Scholar 

  3. Zhang D, Zhu M, Zhao L, Zhang J, Wang K, Qi D, Zhou Y, Bian Y, Jiang J (2017) Ratiometric fluorescent detection of Pb(2+) by FRET-based Phthalocyanine-Porphyrin dyads. Inorg Chem 56(23):14533–14539

    Article  CAS  Google Scholar 

  4. Kim JH, Han SH, Chung BH (2011) Improving Pb2+ detection using DNAzyme-based fluorescence sensors by pairing fluorescence donors with gold nanoparticles. Biosens Bioelectron 26(5):2125–2129

    Article  CAS  Google Scholar 

  5. Khan NA, Niaz A, Zaman MI, Khan FA, Nisar-ul-haq M, Tariq M (2018) Sensitive and selective colorimetric detection of Pb2+ by silver nanoparticles synthesized from Aconitum violaceum plant leaf extract. Mater Res Bull 102:330–336

    Article  Google Scholar 

  6. Choudhury R, Misra TK (2018) Gluconate stabilized silver nanoparticles as a colorimetric sensor for Pb 2+. Colloid  Surface A 545:179–183

    Article  CAS  Google Scholar 

  7. Zhang B, Wei C (2018) Highly sensitive and selective detection of Pb 2+ using a turn-on fluorescent aptamer DNA silver nanoclusters sensor. Talanta 182:125–130

    Article  CAS  Google Scholar 

  8. Zhang D, Zhu M, Zhao L, Zhang J, Wang K, Qi D, Zhou Y, Bian Y, Jiang J (2017) Ratiometric fluorescent detection of Pb2+ by FRET-based Phthalocyanine-Porphyrin dyads. Inorg Chem 56(23):14533–14539

    Article  CAS  Google Scholar 

  9. Ouyang H, Ling S, Liang A, Jiang Z (2018) A facile aptamer-regulating gold nanoplasmonic SERS detection strategy for trace lead ions. Sensors Actuators B Chem 258:739–744

    Article  CAS  Google Scholar 

  10. Yu X, Aijun T, Yi L (2009) Abasic site-containing DNAzyme and aptamer for label-free fluorescent detection of Pb(2+) and adenosine with high sensitivity, selectivity, and tunable dynamic range. J Am Chem Soc 131(42):15352–15357

    Article  Google Scholar 

  11. Shenshan Z, Yuangen W, Yanfang L, Le L, Lan H, Haibo X, Pei Z (2014) Label-free fluorescent sensor for lead ion detection based on lead(II)-stabilized G-quadruplex formation. Anal Biochem 462:19–25

    Article  Google Scholar 

  12. Mou X, Wang J, Meng X, Liu J, Shi L, Sun L (2017) Multifunctional nanoprobe based on upconversion nanoparticles for luminescent sensing and magnetic resonance imaging. J Lumin 190:16–22

    Article  CAS  Google Scholar 

  13. Wu B, Cao Z, Zhang Q, Wang G (2018) NIR-responsive DNA hybridization detection by high efficient FRET from 10-nm upconversion nanoparticles to SYBR green I. Sensors Actuators B Chem 255:2853–2860

    Article  CAS  Google Scholar 

  14. Ouyang Q, Liu Y, Chen Q, Guo Z, Zhao J, Li H, Hu W (2017) Rapid and specific sensing of tetracycline in food using a novel upconversion aptasensor. Food Control 81:156–163

    Article  CAS  Google Scholar 

  15. Li H, Ahmad W, Rong Y, Chen Q, Zuo M, Ouyang Q, Guo Z (2020) Designing an aptamer based magnetic and upconversion nanoparticles conjugated fluorescence sensor for screening Escherichia coli in food. Food Control 107:106761

    Article  CAS  Google Scholar 

  16. Chen M, Kutsanedzie FYH, Cheng W, Agyekum AA, Li H, Chen Q (2018) A nanosystem composed of upconversion nanoparticles and N, N-diethyl-p-phenylenediamine for fluorimetric determination of ferric ion. Microchim Acta 185(8):378

  17. Chen M, Kutsanedzie FYH, Cheng W, Li H, Chen Q (2019) Ratiometric fluorescence detection of Cd2+ and Pb2+ by inner filter-based upconversion nanoparticle-dithizone nanosystem. Microchem J 144:296–302

    Article  CAS  Google Scholar 

  18. Li X, Wu Y, Liu Y, Zou X, Yao L, Li F, Feng W (2014) Cyclometallated ruthenium complex-modified upconversion nanophosphors for selective detection of Hg2+ ions in water. Nanoscale 6(2):1020–1028

    Article  CAS  Google Scholar 

  19. Ding N, Cao Q, Zhao H, Yang Y, Zeng L, He Y, Xiang K, Wang G (2010) Colorimetric assay for determination of lead (II) based on its incorporation into gold nanoparticles during their synthesis. Sensors-Basel  10(12):11144–11155

    Article  CAS  Google Scholar 

  20. Zhang H, Zhang Y, Jin R, Wu C, Zhang B, Zhang Q, Chen X (2018) Preparation and photothermal therapy of hyaluronic acid–conjugated au nanoparticle-coated poly (glycidyl methacrylate) nanocomposites. J Mater Sci 53:16252–16262

    Article  CAS  Google Scholar 

  21. Dai D, Xu D, Cheng X, He Y (2014) Direct imaging of single gold nanoparticle etching: sensitive detection of lead ions. Anal Methods 6(13):4507–4511

    Article  CAS  Google Scholar 

  22. Mou Y, Yang H, Xu ZL (2017) Morphology, surface layer evolution and structure-dye adsorption relationship of porous Fe3O4 MNPs prepared by solvothermal/gas generation process. ACS Sustain Chem Eng 5(3):2339–2349

    Article  CAS  Google Scholar 

  23. Demin AM, Mekhaev AV, Esin AA, Kuznetsov DK, Zelenovskiy PS, Shur VY, Krasnov VP (2018) Immobilization of PMIDA on Fe 3 O 4 magnetic nanoparticles surface: mechanism of bonding. Appl Surf Sci 440:1196–1203

    Article  CAS  Google Scholar 

  24. Singh P, Upadhyay C (2018) Role of silver Nanoshells on structural and magnetic behavior of Fe 3 O 4 nanoparticles. J Magn Magn Mater 458:39–47

    Article  CAS  Google Scholar 

  25. Zhao Y, Xie X, Zhao Y, Xie X (2017) A novel electrochemical Aptamer biosensor based on DNAzyme decorated au@Ag Core-Shell nanoparticles for Hg2+ determination. J Braz Chem Soc 29(2):232–239

    Google Scholar 

  26. Wang Q, Yang XH, Wang L, Wang KM, Zhao X (2007) Novel fluorescent probe for lead ion detection based on DNAzyme. Chem J Chin Univ 28(12):2270–2273

    CAS  Google Scholar 

  27. Chen Q, Hu W, Sun C, Li H, Ouyang Q (2016) Synthesis of improved upconversion nanoparticles as ultrasensitive fluorescence probe for mycotoxins. Anal Chim Acta 938:137–145

    Article  CAS  Google Scholar 

  28. Hu W, Chen Q, Li H, Ouyang Q, Zhao J (2016) Fabricating a novel label-free aptasensor for acetamiprid by fluorescence resonance energy transfer between NH2-NaYF4: Yb, Ho@SiO2 and Au nanoparticles. Biosens Bioelectron 80:398–404

    Article  CAS  Google Scholar 

  29. Li H, Chen Q, Hassan MM, Ouyang Q, Jiao T, Xu Y, Chen M (2018) AuNS@Ag core-shell nanocubes grafted with rhodamine for concurrent metal-enhanced fluorescence and surfaced enhanced Raman determination of mercury ions. Anal Chim Acta 1018:94–103

    Article  CAS  Google Scholar 

  30. Shijia W, Nuo D, Zhouping W, Hongxin W (2011) Aptamer-functionalized magnetic nanoparticle-based bioassay for the detection of ochratoxin a using upconversion nanoparticles as labels. Analyst 136(11):2306–2314

    Article  Google Scholar 

  31. Liu Y, Ouyang Q, Li H, Chen M, Zhang Z, Chen Q (2018) Turn-on Fluoresence sensor for Hg2+ in food based on FRET between Aptamers-functionalized Upconversion nanoparticles and gold nanoparticles. J Agric Food Chem 60:6188–6195

    Article  Google Scholar 

  32. Erdemoǧlu SB, Pyrzyniska K, Güçer Ş (2000) Speciation of aluminum in tea infusion by ion-exchange resins and flame AAS detection. Anal Chim Acta 411(1):81–89

    Article  Google Scholar 

  33. Liu Y, Ouyang Q, Li H, Zhang Z, Chen Q (2017) Development of an inner filter effects-based Upconversion nanoparticles-Curcumin Nanosystem for the sensitive sensing of fluoride ion. ACS Appl Mater Interfaces 9(21):18314–18321

    Article  CAS  Google Scholar 

  34. Sun C, Li H, Koidis A, Chen Q (2016) Quantifying Aflatoxin B1 in peanut oil using fabricating fluorescence probes based on upconversion nanoparticles. Spectrochimica acta. Spectrochim Acta B 165:120–126

    Article  CAS  Google Scholar 

  35. Shahdordizadeh M, Yazdian-Robati R, Ansari N, Ramezani M, Abnous K, Taghdisi SM (2018) An aptamer-based colorimetric lead(II) assay based on the use of gold nanoparticles modified with dsDNA and exonuclease I. Microchim Acta 185(2):151

    Article  Google Scholar 

  36. Chai F, Wang C, Wang T, Li L, Su Z (2010) Colorimetric detection of Pb2+ using glutathione functionalized gold nanoparticles. ACS Appl Mater Interfaces 2(5):1466–1470

    Article  CAS  Google Scholar 

  37. Wang ZX, Yu XH, Li F, Kong FY, Lv WX, Fan DH, Wang W (2017) Preparation of boron-doped carbon dots for fluorometric determination of Pb(II), cu(II) and pyrophosphate ions. Microchim Acta 184(12):4775–4783

    Article  CAS  Google Scholar 

Download references

Acknowlegements

This research is supported by the National Natural Science Foundation of China (31972154, 31901772), the 333 High-level Talents Project of Jiangsu Province (BRA2019087), the Key R&D Program of Jiangsu Province (BE2017357), and the China Postdoctoral Science Foundation (2019 M651748).

Author information

Authors and Affiliations

  1. School of Food and Biological Engineering Jiangsu University, Xuefu Road 301, Zhenjiang, 212013, People’s Republic of China

    Min Chen, Mehedi Hassan, Huanhuan Li & Quansheng Chen

Authors
  1. Min Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mehedi Hassan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Huanhuan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Quansheng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Huanhuan Li or Quansheng Chen.

Ethics declarations

The author(s) declare that they have no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(DOCX 606 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, M., Hassan, M., Li, H. et al. Fluorometric determination of lead(II) by using aptamer-functionalized upconversion nanoparticles and magnetite-modified gold nanoparticles. Microchim Acta 187, 85 (2020). https://doi.org/10.1007/s00604-019-4030-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00604-019-4030-4

Keywords

Search

Navigation