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Electrospun core-shell nanofibers as an adsorbent for on-line micro-solid phase extraction of monohydroxy derivatives of polycyclic aromatic hydrocarbons from human urine, and their quantitation by LC-MS

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Abstract

A method is described for automatted on-line micro-solid phase extraction using polydopamine-modified polystyrene/silica electrospun nanofibers as an adsorbent for monohydroxy metabolites of polycyclic aromatic hydrocarbons (OH-PAHs). They were then quantified by liquid chromatography-mass spectrometry. The sorbent has a network structure, high porosity, stability, hydrophilicity and good extraction capacity. Under optimal experimental conditions, the method has (a) wide linear ranges (0.03–25 ng·mL−1 for 3-hydroxyphenanthrene and 1-hydroxypyrene; and 0.05–25 ng·mL−1 for 2-naphthol), (b) good precision (with relative standard deviations from 2.0 to 12.4%), (c) high enrichment factors (24–112), (d) low limits of detection (2–21 pg·mL−1), and (e) acceptable recoveries from spiked samples (79.1 to 108%). The method was successfully applied for the analysis of trace OH-PAHs in urine.

Schematic illustration of online micro-solid phase extraction using polydopamine-modified polystyrene/silica electrospun nanofibers as adsorbent for the enrichment and determination of monohydroxy metabolites of polycyclic aromatic hydrocarbons (OH-PAHs) prior to LC-MS analysis.

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Acknowledgments

This research was financially supported by National Natural Science Foundation of China (No. 21675058), Hubei Province Natural Science Fund Project outstanding youth project (No. 2017CFA075), the Fundamental Research Funds for the Central Universities (No. CCNU18TS006), International Joint Research Center for Intelligent Biosensing Technology and Health and the 111 Project B17019.

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  1. Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079, People’s Republic of China

    Dan Chen & Hui Xu

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  1. Dan Chen
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  2. Hui Xu
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Correspondence to Hui Xu.

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Chen, D., Xu, H. Electrospun core-shell nanofibers as an adsorbent for on-line micro-solid phase extraction of monohydroxy derivatives of polycyclic aromatic hydrocarbons from human urine, and their quantitation by LC-MS. Microchim Acta 187, 57 (2020). https://doi.org/10.1007/s00604-019-4007-3

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