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Colorimetric determination of the activities of tyrosinase and catalase via substrate-triggered decomposition of MnO2 nanosheets

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Abstract

The authors describe novel colorimetric assays for tyrosinase (TYR) and catalase (CAT) based on the substrate-triggered decomposition of MnO2 nanosheets (NSs). The MnO2 NSs can act as oxidase mimics that catalyze the oxidation of the substrate tetramethylbenzidine (TMB) to form a blue dye with an absorption maximum at 652 nm. The oxidase-mimicking activity of the MnO2 NSs is inhibited by dopamine (DA)/hydrogen peroxide (H2O2) due to their decomposition of the MnO2 NSs. TYR catalyzes the oxidation of DA while CAT can decompose H2O2 into water and oxygen. Therefore, the oxidase-mimicking activity of MnO2 NSs is restored in the presence of both enzymes and their substrates. Based on the competitive consumption of substrates between enzymes and MnO2 NSs, a colorimetric method for determination of enzyme activity and its substrate is developed. The detection limits for TYR and CAT are 6 mU·mL−1 and 33 mU·mL−1, respectively.

A colorimetric method for monitoring enzyme activity and its substrate is described. It is based on the substrate-inhibited oxidase-mimicking activity of MnO2 nanosheets.

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Acknowledgements

This work is kindly supported by the National Natural Science Foundation of China (Nos. 21405094, 21775088, and 81403051), the Natural Science Foundation of Qinghai Province of China (2016-ZJ-955), and the Development Project of Qinghai Key Laboratory (No. 2017-ZJ-Y10), and the Student Research Training Program of Qufu Normal University (2018A055).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu City, 273165, Shandong Province, China

    Xian-En Zhao, Ya-Nan Zuo, Xiaoqing Qu, Lingyuan Liu & Shuyun Zhu

  2. Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai Province, Xining City, 810001, Qinghai, China

    Jing Sun

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  1. Xian-En Zhao
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  2. Ya-Nan Zuo
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Correspondence to Shuyun Zhu.

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Zhao, XE., Zuo, YN., Qu, X. et al. Colorimetric determination of the activities of tyrosinase and catalase via substrate-triggered decomposition of MnO2 nanosheets. Microchim Acta 186, 848 (2019). https://doi.org/10.1007/s00604-019-3995-3

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