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A monolithic capillary modified with a copoplymer prepared from the ionic liquid 1-vinyl-3-octylimidazolium bromide and styrene for electrochromatography of alkylbenzenes, polycyclic aromatic hydrocarbons, proteins and amino acids

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Abstract

A monolithic capillary column was prepared by single-step copolymerization of the ionic liquid 1-vinyl-3-octylimidazolium bromide (VOI) and styrene. The VOI and styrene monomers were introduced to provide multiple interaction sites and increase hydrophobicity and aromaticity of the monolithic column, respectively. The effect of porogen ratio, monomer ratio and reaction temperature on permeability was investigated. The resulting column was characterized by scanning electron microscopy, and the results suggest that the column possesses high porosity and good homogeneity. A relatively strong anodic electroosmotic flow was generated over a wide range of pH values (pH 2.0–10.0), and this facilitates the rapid separation of analytes within 12 min. Alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs), proteins and amino acids were used to evaluate the performance of the monolithic column under capillary electrochromatography mode by UV detection. Mixed-mode retention mechanisms including hydrophobic interaction, π-stacking, ion-exclusion interaction were observed. The monolithic column exhibits high column efficiency (8.72 × 104 plates∙m−1) and satisfying separation capability (the resolution of four alkylbenzenes: 2.54, 2.86 and 4.62, four PAHs: 2.79, 4.83 and 4.77, three proteins: 4.35 and 2.86, two amino acids: 3.34).

Schematic representation of capillary electrochromatography (CEC) systems with ionic liquid and styrene based organic polymer monolithic column for separation of alkylbenzenes, polycyclic aromatic hydrocarbons, proteins and amino acids.

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Acknowledgements

This work was supported by the Natural Science Foundation of Jiangsu Province (Program No.: BK20141353).

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Authors and Affiliations

  1. Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, No.24 Tongjiaxiang, Nanjing, Jiangsu, 210009, People’s Republic of China

    Ying Xi, Yingxiang Du, Xiaodong Sun, Shiyuan Zhao, Zijie Feng, Cheng Chen & Wen Ding

  2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, People’s Republic of China

    Ying Xi, Yingxiang Du, Xiaodong Sun, Shiyuan Zhao, Zijie Feng, Cheng Chen & Wen Ding

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  1. Ying Xi
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Xi, Y., Du, Y., Sun, X. et al. A monolithic capillary modified with a copoplymer prepared from the ionic liquid 1-vinyl-3-octylimidazolium bromide and styrene for electrochromatography of alkylbenzenes, polycyclic aromatic hydrocarbons, proteins and amino acids. Microchim Acta 187, 67 (2020). https://doi.org/10.1007/s00604-019-3894-7

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