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Ultrasensitive ciprofloxacin assay based on the use of a fluorescently labeled aptamer and a nanocomposite prepared from carbon nanotubes and MoSe2

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Abstract

A nanocomposite was prepared from carbon nanotubes and MoSe2 (CNT-MoSe2). This nanomaterial quenches the fluorescence of fluorescein-labeled aptamers. When ciprofloxacin (CIP) binds to the aptamer, an aptamer/G-quadruplex complex will be formed and the interaction between labeled aptamer and CNT-MoSe2 nanostructures is weakened. This leads to significant fluorescence recovery. Under optimized experimental conditions, the limit of detection is 0.63 ng mL−1 with a good linearity in the range from 0.63 to 80 ng mL−1. The assay was applied to the determination of CIP in spiked milk, and the recoveries range between 94.3 and 97.0% (n = 3). Conceivably, the method is a generic approach that can be extended to the determination of other analyte for which adequate aptamers are available.

Schematic presentation of CNT-MoSe2 quenching based aptamer assay for the detection of ciprofloxacin. The assay exhibits good selectivity, stability and reproducibility, and low limit of detection.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (61471233, 51590902). Gaoyuan Discipline of Shanghai-Environmental Science and Engineering (Resource Recycling Science and Engineering).

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Author notes

  1. Xiaobing Hu and Pengju Wei contributed equally to this work.

Authors and Affiliations

  1. School of Environmental and Materials Engineering, College of Engineering, Shanghai Polytechnic University, Shanghai, 201209, China

    Xiaobing Hu, Pengju Wei, Zhanhong Li & Zhigang Zhu

  2. School of Microelectronics, Fudan University, Shanghai, 200433, China

    Xiaobing Hu

  3. BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860, Perpignan, France

    Gaëlle Catanante & Jean Louis Marty

  4. Research Center of Resource Recycling Science and Engineering, Shanghai Ploytechnic University, Shanghai, 201209, China

    Zhigang Zhu

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  1. Xiaobing Hu
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  3. Gaëlle Catanante
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  4. Zhanhong Li
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Correspondence to Zhigang Zhu.

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Hu, X., Wei, P., Catanante, G. et al. Ultrasensitive ciprofloxacin assay based on the use of a fluorescently labeled aptamer and a nanocomposite prepared from carbon nanotubes and MoSe2. Microchim Acta 186, 507 (2019). https://doi.org/10.1007/s00604-019-3629-9

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