Light-harvesting metal-organic framework nanoprobes for ratiometric fluorescence energy transfer-based determination of pH values and temperature
- 127 Downloads
Light-harvesting nanoprobes were developed by self-assembly of nanoscale metal-organic frameworks (NMOFs) and stimuli-responsive polymers for fluorometric sensing of pH values and temperature. Two kinds of fluorescent NMOFs (acting as the energy donor) and stimuli-responsive polymers conjugated to fluorophores (acting as energy acceptors) were prepared and characterized. The NMOFs include zirconium(IV) and π-conjugated dicarboxylate ligands. The fluorophores inclued cyaine dyes and a Bodipy dye. The energy donor and energy acceptor form a Förster resonance energy transfer (FRET) nanosystem. In the light-harvesting system, the chain lengths of the stimuli-responsive polymers vary when the local pH value or temperature change. Ratiometric sensing of pH and temperature was accomplished by monitoring fluorescence. pH values were can be sensed between 3.0 and 8.0 under 420 nm excitation and by ratioing the emission peaks at 645 and 530 nm. Temperature can be sensed in the range from 25 to 50 °C under 550 nm excitation and by ratioing the emission peaks at 810 and 695 nm. The nanoprobes display excellent water dispersibility and cell membrane permeability. They were applied to image pH values and temperature in HeLa cells.
KeywordsLight-harvesting MOF Ratiometric Energy transfer pH sensing Temperature sensing
This work was supported by the Macao Science and Technology Development Fund under Grant No.: 052/2015/A2 and 082/2016/A2; the Research Grant of University of Macau under grant No.: MYRG2016-00058-FHS and MYRG2017-00066-FHS.
Compliance with ethical standards
The author(s) declare that they have no competing interests.
- 7.Li J, Rao J, Pu K (2018) Recent progress on semiconducting polymer nanoparticles for molecular imaging and cancer phototherapy. Biomaterials 155:217–235. https://doi.org/10.1016/j.biomaterials.2017.11.025 CrossRefPubMedGoogle Scholar
- 14.Duan X, Chan C, Guo N, Han W, Weichselbaum RR, Lin W (2016) Photodynamic therapy mediated by nontoxic Core-Shell nanoparticles synergizes with immune checkpoint blockade to elicit antitumor immunity and Antimetastatic effect on breast Cancer. J Am Chem Soc 138(51):16686–16695. https://doi.org/10.1021/jacs.6b09538 CrossRefPubMedPubMedCentralGoogle Scholar
- 16.Poon C, Duan X, Chan C, Han W, Lin W (2016) Nanoscale coordination polymers Codeliver carboplatin and gemcitabine for highly effective treatment of platinum-resistant ovarian Cancer. Mol Pharm 13(11):3665–3675. https://doi.org/10.1021/acs.molpharmaceut.6b00466 CrossRefPubMedPubMedCentralGoogle Scholar
- 18.He C, Duan X, Guo N, Chan C, Poon C, Weichselbaum RR, Lin W (2016) Core-shell nanoscale coordination polymers combine chemotherapy and photodynamic therapy to potentiate checkpoint blockade cancer immunotherapy. Nat Commun 7:12499. https://doi.org/10.1038/ncomms12499 CrossRefPubMedPubMedCentralGoogle Scholar
- 26.van der Meer BW (2014) Forster theory. In: Medintz I, Hildebrandt N (eds) FRET—Forster resonance energy transfer: from theory to applications. 1st ed. Wiley-VCH Verlag GmbH & co. KGaA, pp 23–62Google Scholar
- 29.Ding Z, Cao X (2013) Affinity precipitation of human serum albumin using a thermo-response polymer with an L-thyroxin ligand. BMC Biotechnol 13(1):109. https://doi.org/10.1186/1472-6750-13-109