Skip to main content
SpringerLink
Log in

Colorimetric aggregation assay for kanamycin using gold nanoparticles modified with hairpin DNA probes and hybridization chain reaction-assisted amplification

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

The authors describe a colorimetric method for determination of kanamycin by using gold nanoparticles (AuNPs) as the element of signal-conversion and by applying hybridization chain reaction-assisted signal amplification. The assay is carried out by monitoring the absorbance change and color change adding salt to the reaction solution containing kanamycin (analyte), hairpin DNA probe, and AuNPs. Three hairpin DNA probes with sticky ends were absorbed on the AuNPs via their sticky ends. Cating with DNA prevents them from salt-induced aggregation (which leads to a color change from red to blue) in the complete absence of kanamycin. In contrast, in the presence of kanamycin, the aptamer hairpin DNA probe binds kanamycin, and the newly exposed section of DNA triggers a cascade of hybridization chain reactions with formation of numerous dsDNAs. On addition of salt, the AuNPs form blue aggregates due to the repulsion between dsDNA and AuNPs. Under optimal conditions, the ration of absorbance at 520 and 630 nm drops with the kanamycin concentration in the range from 1 to 40 μM, and the limit of detection is 0.68 μM. The assay can selectively distinguish kanamycin from other antibiotics. The method was applied to kanamycin detection in (spiked) milk samples and gave excellent recoveries.

Schematic presentation of colorimetric method for kanamycin detection using gold nanoparticles modified with hairpin DNA probes and hybridization chain reaction-assisted amplification.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Schemes 1
Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Qin L, Zeng G, Lai C, Huang D, Zhang C, Xu P, Hu T, Liu X, Cheng M, Liu Y (2017) A visual application of gold nanoparticles: simple, reliable and sensitive detection of kanamycin based on hydrogen-bonding recognition. Sensors Actuators B Chem 243:946–954

    Article  CAS  Google Scholar 

  2. Ma L, Sun N, Tu C, Zhang Q, Diao A (2017) Design of an aptamer–based fluorescence displacement biosensor for selective and sensitive detection of kanamycin in aqueous samples. RSC Adv 7(61):38512–38518

    Article  CAS  Google Scholar 

  3. Liu J, Zeng J, Tian Y, Zhou N (2018) An aptamer and functionalized nanoparticle-based strip biosensor for on-site detection of kanamycin in food samples. Analyst 143(1):182–189

    Article  CAS  Google Scholar 

  4. Zhu Y, Chandra P, Song K-M, Ban C, Shim Y-B (2012) Label-free detection of kanamycin based on the aptamer-functionalized conducting polymer/gold nanocomposite. Biosens Bioelectron 36(1):29–34

    Article  CAS  Google Scholar 

  5. Blanchaert B, Jorge EP, Jankovics P, Adams E, Van Schepdael A (2013) Assay of kanamycin a by HPLC with direct UV detection. Chromatographia 76(21–22):1505–1512

    Article  CAS  Google Scholar 

  6. Zhang Y, He HM, Zhang J, Liu FJ, Li C, Wang BW, Qiao RZ (2015) HPLC-ELSD determination of kanamycin B in the presence of kanamycin a in fermentation broth. Biomed Chromatogr 29(3):396–401

    Article  CAS  Google Scholar 

  7. Pikkemaat MG (2009) Microbial screening methods for detection of antibiotic residues in slaughter animals. Anal Bioanal Chem 395(4):893–905

    Article  CAS  Google Scholar 

  8. Long Y, Hernandez M, Kaale E, Van Schepdael A, Roets E, Borrull F, Calull M, Hoogmartens J (2003) Determination of kanamycin in serum by solid-phase extraction, pre-capillary derivatization and capillary electrophoresis. J Chromatogr B 784(2):255–264

    Article  CAS  Google Scholar 

  9. Wei Q, Zhao Y, Du B, Wu D, Li H, Yang M (2012) Ultrasensitive detection of kanamycin in animal derived foods by label-free electrochemical immunosensor. Food Chem 134(3):1601–1606

    Article  CAS  Google Scholar 

  10. Qin X, Yin Y, Yu H, Guo W, Pei M (2016) A novel signal amplification strategy of an electrochemical aptasensor for kanamycin, based on thionine functionalized graphene and hierarchical nanoporous PtCu. Biosens Bioelectron 77:752–758

    Article  CAS  Google Scholar 

  11. Long F, Zhang Z, Yang Z, Zeng J, Jiang Y (2015) Imprinted electrochemical sensor based on magnetic multi-walled carbon nanotube for sensitive determination of kanamycin. J Electroanal Chem 755:7–14

    Article  CAS  Google Scholar 

  12. Li H, D-e S, Liu Y, Liu Z (2014) An ultrasensitive homogeneous aptasensor for kanamycin based on upconversion fluorescence resonance energy transfer. Biosens Bioelectron 55:149–156

    Article  CAS  Google Scholar 

  13. Li C, Zhang Y, Eremin SA, Yakup O, Yao G, Zhang X (2017) Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIA. Food Chem 227:48–54

    Article  CAS  Google Scholar 

  14. Chen Y, Wang Z, Wang Z, Tang S, Zhu Y, Xiao X (2008) Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for kanamycin and tobramycin in swine tissues. J Agric Food Chem 56(9):2944–2952

    Article  CAS  Google Scholar 

  15. Xu Y, Han T, Li X, Sun L, Zhang Y, Zhang Y (2015) Colorimetric detection of kanamycin based on analyte-protected silver nanoparticles and aptamer-selective sensing mechanism. Anal Chim Acta 891:298–303

    Article  CAS  Google Scholar 

  16. Zeng S, Yong K-T, Roy I, Dinh X-Q, Yu X, Luan F (2011) A review on functionalized gold nanoparticles for biosensing applications. Plasmonics 6(3):491–506

    Article  CAS  Google Scholar 

  17. Liu C-W, Hsieh Y-T, Huang C-C, Lin Z-H, Chang H-T (2008) Detection of mercury (II) based on hg 2+–DNA complexes inducing the aggregation of gold nanoparticles. Chem Commun (19):2242–2244

  18. Sabela M, Balme S, Bechelany M, Janot JM, Bisetty K (2017) A review of gold and silver nanoparticle-based colorimetric sensing assays. Adv Eng Mater 19(12):1700270

    Article  Google Scholar 

  19. Priyadarshini E, Pradhan N (2017) Gold nanoparticles as efficient sensors in colorimetric detection of toxic metal ions: a review. Sensors Actuators B Chem 238:888–902

    Article  CAS  Google Scholar 

  20. Dirks RM, Pierce NA (2004) Triggered amplification by hybridization chain reaction. Proc Natl Acad Sci U S A 101(43):15275–15278

    Article  CAS  Google Scholar 

  21. Wang X, Jiang A, Hou T, Li H, Li F (2015) Enzyme-free and label-free fluorescence aptasensing strategy for highly sensitive detection of protein based on target-triggered hybridization chain reaction amplification. Biosens Bioelectron 70:324–329

    Article  Google Scholar 

  22. Gao Z, Qiu Z, Lu M, Shu J, Tang D (2017) Hybridization chain reaction-based colorimetric aptasensor of adenosine 5′-triphosphate on unmodified gold nanoparticles and two label-free hairpin probes. Biosens Bioelectron 89:1006–1012

    Article  CAS  Google Scholar 

  23. Liu J, Lu Y (2006) Preparation of aptamer-linked gold nanoparticle purple aggregates for colorimetric sensing of analytes. Nat Protoc 1(1):246–252

    Article  CAS  Google Scholar 

  24. Luo Y, Xu J, Li Y, Gao H, Guo J, Shen F, Sun C (2015) A novel colorimetric aptasensor using cysteamine-stabilized gold nanoparticles as probe for rapid and specific detection of tetracycline in raw milk. Food Control 54:7–15

    Article  CAS  Google Scholar 

  25. Zhou N, Zhang J, Tian Y (2014) Aptamer-based spectrophotometric detection of kanamycin in milk. Anal Methods 6(5):1569–1574

    Article  CAS  Google Scholar 

  26. Wang C, Wang C, Wang Q, Chen D (2017) Resonance light scattering method for detecting kanamycin in milk with enhanced sensitivity. Anal Bioanal Chem 409(11):2839–2846

    Article  CAS  Google Scholar 

  27. Li H, Rothberg L (2004) Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. Proc Natl Acad Sci U S A 101(39):14036–14039

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. 31301468).

Author information

Authors and Affiliations

  1. School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Chengnan Xu, Yibin Ying & Jianfeng Ping

  2. Zhejiang A&F University, Hangzhou, 311300, People’s Republic of China

    Yibin Ying

Authors
  1. Chengnan Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yibin Ying
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jianfeng Ping
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jianfeng Ping.

Ethics declarations

The author(s) declare that they have no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(DOCX 1438 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xu, C., Ying, Y. & Ping, J. Colorimetric aggregation assay for kanamycin using gold nanoparticles modified with hairpin DNA probes and hybridization chain reaction-assisted amplification. Microchim Acta 186, 448 (2019). https://doi.org/10.1007/s00604-019-3574-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00604-019-3574-7

Keywords

Search

Navigation