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Rapid identification and antibiotic susceptibility test of pathogens in blood based on magnetic separation and surface-enhanced Raman scattering

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Abstract

An effective surface-enhanced Raman scattering (SERS) method is presented for the rapid identification and drug sensitivity analysis of pathogens in blood. In a first step, polyethyleneimine-modified magnetic microspheres (Fe3O4@PEI) were used to enrich bacteria from blood samples. Next, the Fe3O4@PEI@bacteria complex was cultured on both ordinary and drug-sensitive plates. Lastly, the SERS spectra of single colonies were acquired in order to identify different pathogens and their resistant strains by comparison with established standardized bacterial SERS spectras and orthogonal partial least squares discriminant analysis (OPLS-DA) method. Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa and their resistant strains were used to evaluate the performance of the SERS method. The results demonstrate that the method can accurately detect and identify all the tested sensitive and drug-resistant strains of bacteria, including 77 clinical blood infection samples. The method provides a way for rapid identification and susceptibility test of pathogens, and has great potential to replace currently used time-consuming methods.

Schematic presentation of a method for the rapid identification and drug sensitivity analysis of pathogens in blood. It is based on a combination of magnetic separation, SERS fingerprint analysis and orthogonal partial least squares discriminant analysis (OPLS-DA).

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No 81471994, 81830101), Jiangsu Provincial Medical Talent (ZDRCA2016053), Six talent peaks project of Jiangsu Province (WSN-135), Advanced health talent of six-one project of Jiangsu Province (LGY2016042), Infectious diseases major project(2018ZX10712001-010).

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Authors and Affiliations

  1. Medical Technology Institute of Xuzhou Medical University, Xuzhou, 221004, People’s Republic of China

    Jia Li, Luoluo Shi, Peiwen Fu, Shengqi Wang & Bing Gu

  2. Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221004, People’s Republic of China

    Jia Li & Bing Gu

  3. Beijing Institute of Radiation Medicine, Beijing, 100850, People’s Republic of China

    Chongwen Wang, Liting Shao, Keli Wang, Rui Xiao & Shengqi Wang

  4. College of Life Sciences, Anhui Agricultural University, Hefei, 230036, People’s Republic of China

    Chongwen Wang

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  1. Jia Li
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Correspondence to Chongwen Wang, Rui Xiao, Shengqi Wang or Bing Gu.

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Li, J., Wang, C., Shi, L. et al. Rapid identification and antibiotic susceptibility test of pathogens in blood based on magnetic separation and surface-enhanced Raman scattering. Microchim Acta 186, 475 (2019). https://doi.org/10.1007/s00604-019-3571-x

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