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In-situ microscale spectrophotometric determination of phenytoin by using branched gold nanoparticles

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Abstract

A rapid method for the sensitive detection of phenytoin (PHT) by branched gold nanoparticles (B-AuNPs) is described. These nanoparticles were synthesized by adding methanol as the reducing agent and poly(ethylene glycol) as the stabilizer at 70 °C. The B-AuNPs are red in color with an absorption maximum at 540 nm when prepared in situ. However, the color becomes increasingly weaker when PHT is present in increasing concentrations. This method can determine PHT over the 67–670 ng·mL−1 concentration range, with detection limit of 21 ng·mL−1. The relative standard deviation for five replicate measurements at 68 and 530 ng·mL−1 of PHT was 3.2% and 1.2%, respectively. The method was applied to the determination of PHT in plasma samples of epileptic patients, and the results were in agreement with those obtained by a standard official method.

Branched gold nanoparticles (AuNPs) prepared in situ have a red color with an absorption maximum at 540 nm. The color becomes increasingly weaker with decreasing the intensity of the characteristic SPR band when PHT is present in increasing concentration. The current assay is capable of determining PHT over the 67–670 ng·mL−1 concentration range with a limit of detection of 21 ng·mL−1.

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Acknowledgements

This work was financially supported by the “Vice Chancellor of Research and Technology, Tabriz University of Medical Sciences” under the grant number of 594252.

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Authors and Affiliations

  1. Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, 51656-65811, Iran

    Maryam Khoubnasabjafari

  2. Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 51656-65811, Iran

    Azam Samadi & Abolghasem Jouyban

  3. Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz, 51656-65811, Iran

    Abolghasem Jouyban

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  1. Maryam Khoubnasabjafari
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  2. Azam Samadi
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  3. Abolghasem Jouyban
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Correspondence to Azam Samadi.

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Khoubnasabjafari, M., Samadi, A. & Jouyban, A. In-situ microscale spectrophotometric determination of phenytoin by using branched gold nanoparticles. Microchim Acta 186, 422 (2019). https://doi.org/10.1007/s00604-019-3546-y

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