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A chemiluminescence resonance energy transfer strategy and its application for detection of platinum ions and cisplatin

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Abstract

A novel chemiluminescence resonance energy transfer (CRET) system was developed and combined with a structure-switching aptamer for the highly sensitive detection of platinum. Platinum was chosen as a model analyte to demonstrate the generality of the new CRET system. This aptameric platform consisted of a streptavidin labeled aptamer against platinum and a streptavidin-coated magnetic bead for the selective separation of platinum-bound aptamer. The platinum–aptamer probe contained several guanine (G) bases bound to the 3,4,5-trimethoxyphenyl-glyoxal (TMPG) donor group at the 5′ end, a fluorescent acceptor (6-carboxy-2′,4,7,7′-tetrachlorofluorescein, TET) at the 3′ end, and a streptavidin aptamer sequence in which several base pairs were replaced by the G-G mismatch to induce the platinum-oligonucleotide coordination. The chemiluminescence (CL) generated by TMPG/G bases is transferred to the acceptor (TET). In the presence of platinum, the platinum–aptamer probe was folded such that the G bases at the 5′ end and TET at the 3′ were in close proximity. The complex was separated using streptavidin-coated magnetic beads by the addition of TMPG to form the TMPG/G bases complex. The ultraweak CL from the TMPG/G bases was strongly enhanced by TET. This novel CRET-based method can be easily performed with high limit of detection (50 ng·mL−1) and selectivity over other metal ions. This technique provides a novel method for simple, fast, and convenient point-of-care diagnostics for monitoring proteins and metal ions.

Schematic presentation of chemiluminescence resonance energy transfer (CRET) detection of platinum(II) by Pt–base pair coordination to the aptamer. TMPG: 3,4,5-trimethoxyphenyl-glyoxal, fluorophore TET: 6-carboxy-2′,4,7,7′-tetrachlorofluorescein.

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Acknowledgments

We acknowledge financial support from the Zhejiang Provincial Natural Science Foundation of China (LY18H300003), the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2018R1C1B6001112), the National Natural Science Foundation of China (21405136, 81673399), the National Key Project of China (2017YFC0908600) and Scientific Research Fund of Zhejiang Provincial Education Department (Y201430444).

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  1. Sheng Cai and Ying Zhou contributed equally to this work.

Authors and Affiliations

  1. Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou, 310058, Zhejiang, China

    Sheng Cai, Jiawei Ye, Ruizhe Chen, Lianli Sun & Su Zeng

  2. School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, 201203, China

    Ying Zhou & Jianzhong Lu

  3. Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea

    Cheulhee Jung

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  1. Sheng Cai
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Correspondence to Cheulhee Jung or Su Zeng.

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Cai, S., Zhou, Y., Ye, J. et al. A chemiluminescence resonance energy transfer strategy and its application for detection of platinum ions and cisplatin. Microchim Acta 186, 463 (2019). https://doi.org/10.1007/s00604-019-3509-3

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