Abstract
The exploration of monohydroxy polycyclic aromatic hydrocarbons and 8-hydroxy-2′-deoxyguanosine (8-OHdG) produced by oxidative stress and DNA damage is a powerful and non-invasive tool to study the health risk of exposure to polycyclic aromatic hydrocarbons (PAHs). A nanocomposite prepared from graphene oxide, poly(3,4-ethylenedioxythiophene) and polypyrrole was electrodeposited on the internal surface of a stainless-steel tube for online in-tube solid phase microextraction (IT-SPME) of 8-OHdG, 3-hydroxyphenanthrene and 1-hydroxypyrene from urine. The coating possesses excellent chemical and mechanical stability, high extraction efficiency, good resistance to matrix interference, and a long lifespan. An online IT-SPME-high performance liquid chromatography-mass spectrometry method was developed for the determination of these three metabolite biomarkers in human urine. Figures of merit include (a) enrichment factors of 30–48; (b) low limits of detection (4–41 pg·mL−1 at S/N = 3); (c) wide linear ranges (0.05–50 ng·mL−1); (d) good recoveries from spiked samples (71.6–109.5%); and (e) acceptable repeatability (2.3–14.6%). The method offers the advantages of low cost, simplicity, sensitivity, rapidity and automation.
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Acknowledgments
This research was financially supported by National Natural Science Foundation of China (No. 21675058), Hubei Province Natural Science Fund Project outstanding youth project (No. 2017CFA075), self-determined research funds of CCNU from the colleges’ basic research and operation of MOE (No. CCNU18TS006), International Joint Research Center for Intelligent Biosensing Technology and Health and the 111 Project B17019.
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Chen, D., Xu, H. Simultaneous HPLC-MS determination of 8-hydroxy-2′-deoxyguanosine, 3-hydroxyphenanthrene and 1-hydroxypyrene after online in-tube solid phase microextraction using a graphene oxide/poly(3,4-ethylenedioxythiophene)/polypyrrole composite. Microchim Acta 186, 300 (2019). https://doi.org/10.1007/s00604-019-3429-2
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DOI: https://doi.org/10.1007/s00604-019-3429-2